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Literature summary extracted from

  • Helting, T.
    Biosynthesis of heparin. Solubilization and partial purification of uridine diphosphate glucuronic acid: acceptor glucuronosyltransferase from mouse mastocytoma (1972), J. Biol. Chem., 247, 4327-4332.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.135 0.00025
-
 UDPglucuronate
-
 Mus musculus
2.4.1.135 0.0091
-
 3-beta-galactosyl-galactose
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.135 membrane bound Mus musculus 16020
-
2.4.1.135 microsome
-
 Mus musculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.135 Mn2+ required Mus musculus
2.4.1.135 Mn2+ 20 mM Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.135 UDPglucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein Mus musculus involved in the biosynthesis of the heparin-polypeptide linkage region ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.135 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.135 partial Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.135 mastocytoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.135 additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.135 additional information galactosyl-beta-1,4-xylose, galactosyl-beta-1,4-glucose is a less effective acceptor substrate Mus musculus ?
-
 ?
2.4.1.135 additional information galactosyl-beta-1,6-galactose is a less effective acceptor substrate Mus musculus ?
-
 ?
2.4.1.135 UDPglucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein involved in the biosynthesis of the heparin-polypeptide linkage region Mus musculus ?
-
 ?
2.4.1.135 UDPglucuronate + 3-beta-D-galactosyl-D-galactose
-
 Mus musculus UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-D-galactose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.135 7.5
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.135 6.5 8.5 about 50% of maximal activity at pH 6.5 and pH 8.5 Mus musculus