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Literature summary extracted from

  • Goldsmith, L.A.
    Sulfhydryl oxidase from rat skin (1987), Methods Enzymol., 143, 510-515.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.2 dithiothreitol at high concentrations Rattus norvegicus
1.8.3.2 iodoacetamide
-
Rattus norvegicus
1.8.3.2 iodoacetic acid
-
Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.2 copper native activity is increased 2fold Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.2 66000
-
gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.2
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.3.2 skin
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.3.2 additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 2-mercaptoethanol + O2 3.7% of the activity with dithiothreitol Rattus norvegicus ? + H2O
-
?
1.8.3.2 5,5'-dithiobis(2-nitrobenzoic acid) + O2
-
Rattus norvegicus ? + H2O
-
?
1.8.3.2 D-penicillamine + O2 33% of the activity with dithiothreitol Rattus norvegicus ? + H2O
-
?
1.8.3.2 dithioerythritol + O2
-
Rattus norvegicus ? + H2O
-
?
1.8.3.2 dithiothreitol + O2
-
Rattus norvegicus ? + H2O
-
?
1.8.3.2 GSH + O2 + O2 0.7% of the activity with dithiothreitol Rattus norvegicus GSSG + H2O
-
?
1.8.3.2 L-Cys + O2 17% of the activity with dithiothreitol Rattus norvegicus ? + H2O
-
?
1.8.3.2 N-acetylcysteine + O2 4.6% of the activity with dithiothreitol Rattus norvegicus ? + H2O
-
?
1.8.3.2 reductively denatured ribonuclease A + O2
-
Rattus norvegicus renatured ribonuclease + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.8.3.2 monomer 1 * 66000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.3.2 additional information
-
dithiothreitol, 1 mM, increases the stability to heating Rattus norvegicus
1.8.3.2 60
-
15 min, 85% loss of activity. 30 min, complete loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.2 8 8.2 oxidation of 5,5'-dithiobis(2-nitrobenzoic acid) and reactivation of ribonuclease Rattus norvegicus