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Purification and properties of sulfite oxidase from chicken liver. Presence of molybdenum in sulfite oxidase from diverse sources

Kessler, D.L.; Rajagopalan, K.V.; J. Biol. Chem. 247, 6566-6573 (1972)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.3.1
0.002
-
cytochrome c
-
Gallus gallus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.3.1
Mo
-
Gallus gallus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.3.1
55000
-
? * 55000, SDS-PAGE, heterogenous behaviour in sedimentation equilibrium experiments
Gallus gallus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.3.1
Gallus gallus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.3.1
-
Gallus gallus
Reaction
EC Number
Reaction
Commentary
Organism
1.8.3.1
sulfite + O2 + H2O = sulfate + H2O2
ping-pong mechanism
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.3.1
sulfite + cytochrome c
-
393013
Gallus gallus
sulfate + reduced cytochrome c
-
-
-
-
1.8.3.1
sulfite + H2O + A
A: electron acceptor, i.e. O2, cytochrome c, K3[Fe(CN)6], 2,6-dichloroindophenol, methylene blue, highly specific for sulfite as electron donor
393013
Gallus gallus
SO42- + AH2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.3.1
?
? * 55000, SDS-PAGE, heterogenous behaviour in sedimentation equilibrium experiments
Gallus gallus
1.8.3.1
dimer
-
Gallus gallus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.3.1
heme
-
Gallus gallus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.3.1
heme
-
Gallus gallus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.3.1
0.002
-
cytochrome c
-
Gallus gallus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.3.1
Mo
-
Gallus gallus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.3.1
55000
-
? * 55000, SDS-PAGE, heterogenous behaviour in sedimentation equilibrium experiments
Gallus gallus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.3.1
-
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.3.1
sulfite + cytochrome c
-
393013
Gallus gallus
sulfate + reduced cytochrome c
-
-
-
-
1.8.3.1
sulfite + H2O + A
A: electron acceptor, i.e. O2, cytochrome c, K3[Fe(CN)6], 2,6-dichloroindophenol, methylene blue, highly specific for sulfite as electron donor
393013
Gallus gallus
SO42- + AH2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.3.1
?
? * 55000, SDS-PAGE, heterogenous behaviour in sedimentation equilibrium experiments
Gallus gallus
1.8.3.1
dimer
-
Gallus gallus