BRENDA - Enzyme Database show

Studies on yeast sulfite reductase. I. Purification and characterization

Yoshimoto, A.; Sato, R.; Biochim. Biophys. Acta 153, 555-575 (1968)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.2
2'-AMP
NADPH-sulfite reductase and NADPH-cytochrome c reductase
Saccharomyces cerevisiae
1.8.1.2
arsenite
-
Saccharomyces cerevisiae
1.8.1.2
CN-
only when added with NADPH prior to substrate, sulfite prevents NADPH-reduced form from irreversible conversion by CN-; severe inhibition of NADPH-sulfite,-nitrite,-hydroxylamine reductase, no inhibition of methyl viologen-sulfite reductase, small extent of inhibition of NADPH dependent reduction of cytochrome c, ferricyanide, quinones etc.
Saccharomyces cerevisiae
1.8.1.2
NADP+
NADPH-dependent activities
Saccharomyces cerevisiae
1.8.1.2
p-chloromercuribenzoate
NADPH-dependent activities
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.2
0.012
0.017
sulfite
-
Saccharomyces cerevisiae
1.8.1.2
0.8
1.5
nitrite
-
Saccharomyces cerevisiae
1.8.1.2
4.5
10.5
hydroxylamine
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.1.2
soluble
-
Saccharomyces cerevisiae
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.2
350000
-
calculation from FAD and FMN content, sedimentation coefficient
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.2
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.2
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.2
1.85
-
-
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
1.8.1.2
-20°C, 0.3 M phosphate buffer pH 7.3, 1 year stable stable
Saccharomyces cerevisiae
1.8.1.2
4°C, 0.3 M phosphate buffer pH 7.3, 1 week stable
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.2
hydroxylamine + NADPH
-
392971
Saccharomyces cerevisiae
ammonia + NADP+
-
-
-
-
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
392971
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
nitrite + NADPH
-
392971
Saccharomyces cerevisiae
ammonia + NADP+
-
-
-
?
1.8.1.2
sulfite + NADPH
NADPH can be replaced by reduced methyl viologen
392971
Saccharomyces cerevisiae
sulfide + NADP+ + H2O
-
-
-
?
1.8.1.2
sulfite + NADPH
benzyl viologen
392971
Saccharomyces cerevisiae
sulfide + NADP+ + H2O
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.2
7
7.5
NADPH-sulfite reductase
Saccharomyces cerevisiae
1.8.1.2
7.5
7.9
NADPH-cytochrome c reductase
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.2
2'-AMP
NADPH-sulfite reductase and NADPH-cytochrome c reductase
Saccharomyces cerevisiae
1.8.1.2
arsenite
-
Saccharomyces cerevisiae
1.8.1.2
CN-
only when added with NADPH prior to substrate, sulfite prevents NADPH-reduced form from irreversible conversion by CN-; severe inhibition of NADPH-sulfite,-nitrite,-hydroxylamine reductase, no inhibition of methyl viologen-sulfite reductase, small extent of inhibition of NADPH dependent reduction of cytochrome c, ferricyanide, quinones etc.
Saccharomyces cerevisiae
1.8.1.2
NADP+
NADPH-dependent activities
Saccharomyces cerevisiae
1.8.1.2
p-chloromercuribenzoate
NADPH-dependent activities
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.2
0.012
0.017
sulfite
-
Saccharomyces cerevisiae
1.8.1.2
0.8
1.5
nitrite
-
Saccharomyces cerevisiae
1.8.1.2
4.5
10.5
hydroxylamine
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.1.2
soluble
-
Saccharomyces cerevisiae
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.2
350000
-
calculation from FAD and FMN content, sedimentation coefficient
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.2
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.2
1.85
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.1.2
-20°C, 0.3 M phosphate buffer pH 7.3, 1 year stable stable
Saccharomyces cerevisiae
1.8.1.2
4°C, 0.3 M phosphate buffer pH 7.3, 1 week stable
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.2
hydroxylamine + NADPH
-
392971
Saccharomyces cerevisiae
ammonia + NADP+
-
-
-
-
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
392971
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
nitrite + NADPH
-
392971
Saccharomyces cerevisiae
ammonia + NADP+
-
-
-
?
1.8.1.2
sulfite + NADPH
NADPH can be replaced by reduced methyl viologen
392971
Saccharomyces cerevisiae
sulfide + NADP+ + H2O
-
-
-
?
1.8.1.2
sulfite + NADPH
benzyl viologen
392971
Saccharomyces cerevisiae
sulfide + NADP+ + H2O
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.2
7
7.5
NADPH-sulfite reductase
Saccharomyces cerevisiae
1.8.1.2
7.5
7.9
NADPH-cytochrome c reductase
Saccharomyces cerevisiae