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Literature summary extracted from

  • Sundquist, A.R.; Fahey, R.C.
    The function of gamma-glutamylcysteine and bis-gamma-glutamylcystine reductase in Halobacterium halobium (1989), J. Biol. Chem., 264, 719-725.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.1.13 low-ionic-strength buffer below 1.0 M leads to rapid denaturation Halobacterium salinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.13 AsO2- 25 mM arsenite, relative activity 46% Halobacterium salinarum
1.8.1.13 Cu2+ relative activity less than 5% Halobacterium salinarum
1.8.1.13 Hg2+ relative activity less than 5% Halobacterium salinarum
1.8.1.13 Zn2+ relative activity less than 44% Halobacterium salinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.13 0.29
-
 NADPH
-
 Halobacterium salinarum
1.8.1.13 0.81
-
 bis-gamma-glutamylcystine
-
 Halobacterium salinarum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.13 cytoplasm
-
 Halobacterium salinarum 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.13 61000
-
 2 * 61000, SDS-PAGE Halobacterium salinarum
1.8.1.13 122000
-
-
 Halobacterium salinarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.13 bis-gamma-glutamylcystine + NADPH + H+ Halobacterium salinarum
-
 2 gamma-glutamylcysteine + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.13 Halobacterium salinarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.13
-
 Halobacterium salinarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.13 28
-
-
 Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.13 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
 Halobacterium salinarum 4-nitrobenzenethiol + NADP+
-
 ?
1.8.1.13 bis-gamma-glutamylcystine + NADPH + H+
-
 Halobacterium salinarum 2 gamma-glutamylcysteine + NADP+
-
 ?
1.8.1.13 additional information no activity detected with GSSG Halobacterium salinarum ?
-
 ?
1.8.1.13 additional information lipoic acid disulfide and dihydrolipoamide are no substrates Halobacterium salinarum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.13 dimer 2 * 61000, SDS-PAGE Halobacterium salinarum

Synonyms

EC Number Synonyms Comment Organism
1.8.1.13 GCR
-
 Halobacterium salinarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.13 28.3
-
 FAD
-
 Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.13 7.5
-
-
 Halobacterium salinarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.13 6.3 8.2 about half-maximal activity at pH 6.3 and 8.2 Halobacterium salinarum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.13 FAD flavoprotein, 1 mol FAD/mol subunit Halobacterium salinarum