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Literature summary extracted from

  • Nicholas, D.J.D.; Medina, A.; Jones, O.T.G.
    A nitrite reductase from Neurospora crassa (1960), Biochim. Biophys. Acta, 37, 468-476.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.4 FMN addition increases activity Neurospora crassa
1.7.1.4 pyridoxine stimulates activity in crude extracts from pyridoxine-deficient felts, no activation of purified enzyme Neurospora crassa

General Stability

EC Number General Stability Organism
1.7.1.4 dialysis against water or a variety of buffers deactivates Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.4 1,10-phenanthroline
-
 Neurospora crassa
1.7.1.4 2,2'-bipyridine
-
 Neurospora crassa
1.7.1.4 2-heptyl-4-hydroxyquinoline N-oxide
-
 Neurospora crassa
1.7.1.4 8-hydroxyquinoline
-
 Neurospora crassa
1.7.1.4 Atabrine
-
 Neurospora crassa
1.7.1.4 CN-
-
 Neurospora crassa
1.7.1.4 Diquinolyl
-
 Neurospora crassa
1.7.1.4 EDTA
-
 Neurospora crassa
1.7.1.4 Hydrazine sulfate complete inhibition Neurospora crassa
1.7.1.4 Iodosobenzoate
-
 Neurospora crassa
1.7.1.4 naphthoquinone
-
 Neurospora crassa
1.7.1.4 p-chloromercuribenzoate completely reversed by glutathione Neurospora crassa
1.7.1.4 salicylic acid
-
 Neurospora crassa
1.7.1.4 Sodium diethyldithiocarbamate
-
 Neurospora crassa
1.7.1.4 Thiourea
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.4 0.05
-
 FAD
-
 Neurospora crassa
1.7.1.4 0.05
-
 FMN
-
 Neurospora crassa
1.7.1.4 0.1
-
 NO2-
-
 Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.4 Cu2+ depressed activity in Cu-deficient felt extracts, stimulates activity in extracts from Cu-deficient felts, depresses activity in extracts from control felts Neurospora crassa
1.7.1.4 Fe3+ stimulates activity in Fe-deficient felt extracts Neurospora crassa
1.7.1.4 Magnesium depressed activity in Mg-deficient felt extracts Neurospora crassa
1.7.1.4 Zinc depressed activity in Zn-deficient felt extracts Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.4 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.4
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.4 0.0523
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.7.1.4 -17°C, overnight, 50% loss of activity Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.4 NAD(P)H + nitrite + H+ NADH is preferred Neurospora crassa NAD(P)+ + NH3 + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.4 7.6
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.4 4 11 pH 4.0: 40% activity, pH 11.0: 70% activity Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.4 FAD increases activity Neurospora crassa
1.7.1.4 NADH
-
 Neurospora crassa