Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Prodouz, K.N.; Garrett, R.H.
    Neurospora crassa NAD(P)H-nitrite reductase. Studies on its composition and structure (1981), J. Biol. Chem., 256, 9711-9717.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.4 0.00002
-
 FAD
-
 Neurospora crassa
1.7.1.4 0.0075
-
 NO2-
-
 Neurospora crassa
1.7.1.4 0.015
-
 NADPH
-
 Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.4 Iron 9-10 mol Fe per mol of enzyme, heme iron associated with siroheme moieties, nonheme iron involved in iron-sulfur centers Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.4 140000
-
 2 * 140000, SDS-PAGE Neurospora crassa
1.7.1.4 289400
-
 deduced from amino acid composition Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.4 NAD(P)H + nitrite + H+ Neurospora crassa
-
 NAD(P)+ + NH3 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.4 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.4
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.4 NAD(P)H + nitrite + H+
-
 Neurospora crassa NAD(P)+ + NH3 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.4 dimer 2 * 140000, SDS-PAGE Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.4 FAD exogenously added FAD is required for activity in vitro Neurospora crassa
1.7.1.4 flavin little or no flavin associated with purified protein Neurospora crassa
1.7.1.4 NADPH
-
 Neurospora crassa
1.7.1.4 siroheme
-
 Neurospora crassa