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Literature summary extracted from

  • Jackson, R.H.; Cornish-Bowden, A.; Cole, J.A.
    Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12 (1981), Biochem. J., 193, 861-867.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.7.1.15 1 mM nitrite and 5O mM hydroxylamine are equally effective in decreasing the loss of activity after 24h at 40°C from 40% to 10%. No increase in catalytic activity is detected when enzyme is pre-incubated for 15 min with either of these substrates, even when NAD+ is omitted from the assay mixture Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.15 KCN although 1 mM KCN completely inhibits hydroxylamine reduction, it does not inhibit the reduction of K3Fe(CN)6 and only decreases the rate of cytochrome c reduction by 12% Escherichia coli
1.7.1.15 p-chloromercuribenzoate the reduction of cytochrome c, K3Fe(CN)6 and hydroxylamine is completely inhibited (more than 99%) by 0.02 mM p-chloromercuribenzoate Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.15 88000
-
 x * 88000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.15 nitrite + NADH + H+ Escherichia coli
-
 ammonia + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.15 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.15 purified in the presence of 1 mM NO2- and 0.01 mM FAD by ammonium sulfate precipitation, DEAE-cellulose column chromatography, DEAE-Sephadex gel filtration, and Sephadex G-25 gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.15 0.052
-
 crude extract, at pH 8.0 and 30°C Escherichia coli
1.7.1.15 62.4
-
 after 121fold purification, at pH 8.0 and 30°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.15 horse heart cytochrome c + NADH + H+
-
 Escherichia coli ?
-
 ?
1.7.1.15 K3Fe(CN)6 + NADH + H+
-
 Escherichia coli ?
-
 ?
1.7.1.15 NADH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Escherichia coli NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.7.1.15 nitrite + NADH + H+
-
 Escherichia coli ammonia + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.15 ? x * 88000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.7.1.15 EC 1.6.6.4 formerly Escherichia coli
1.7.1.15 NADH-dependent nitrite reductase
-
 Escherichia coli
1.7.1.15 NADH-nitrite oxidoreductase
-
 Escherichia coli
1.7.1.15 nirB
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.15 FAD the enzyme contains one non-covalently bound FAD molecule Escherichia coli
1.7.1.15 Fe-S center the enzyme contains 5 Fe atoms and 4 acid-labile S atoms per subunit Escherichia coli
1.7.1.15 additional information the enzyme does not contain FMN Escherichia coli
1.7.1.15 NADH
-
 Escherichia coli
1.7.1.15 siroheme
-
 Escherichia coli