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Spectroscopic and kinetic properties of a recombinant form of the flavin domain of spinach NADH:nitrate reductase

Quinn, G.B.; Trimboli, A.J.; Prosser, I.M.; Barber, M.J.; Arch. Biochem. Biophys. 327, 151-160 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.7.1.1
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli
Spinacia oleracea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.1
adenine
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
adenosine
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
adenosine 5'-diphosphoribose
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
ADP
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
AMP
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
ferricyanide
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
NAD+
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
nicotinamide
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
NMN
inhibition of the recombinant FAD domain
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.1.1
additional information
-
additional information
Km-values of the the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli
Spinacia oleracea
1.7.1.1
0.017
-
NADH
recombinant FAD domain
Spinacia oleracea
1.7.1.1
0.034
-
ferricyanide
recombinant FAD domain
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.1.1
additional information
-
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli show a MW of 30000 Da by SDS-PAGE
Spinacia oleracea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.7.1.1
Spinacia oleracea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.7.1.1
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli
Spinacia oleracea
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.1
additional information
-
-
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.1.1
additional information
the isolated flavin-containing domain is capable of reducing cytochrome b5 directly
392916
Spinacia oleracea
?
-
-
-
-
1.7.1.1
nitrate + NADH
-
392916
Spinacia oleracea
nitrite + NAD+ + H2O
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.1.1
6.5
-
-
Spinacia oleracea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.1
NADH
-
Spinacia oleracea
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.7.1.1
0.2
-
ADP
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
0.2
-
ADPribose
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
0.6
-
AMP
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
1.9
-
NAD+
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
4.8
-
adenosine
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
23
-
ferricyanide
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
26
-
adenine
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
62
-
NMN
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
122
-
nicotinamide
inhibition of the recombinant FAD domain
Spinacia oleracea
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.1.1
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli
Spinacia oleracea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.1
NADH
-
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.1
adenine
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
adenosine
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
adenosine 5'-diphosphoribose
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
ADP
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
AMP
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
ferricyanide
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
NAD+
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
nicotinamide
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
NMN
inhibition of the recombinant FAD domain
Spinacia oleracea
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.7.1.1
0.2
-
ADP
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
0.2
-
ADPribose
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
0.6
-
AMP
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
1.9
-
NAD+
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
4.8
-
adenosine
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
23
-
ferricyanide
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
26
-
adenine
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
62
-
NMN
inhibition of the recombinant FAD domain
Spinacia oleracea
1.7.1.1
122
-
nicotinamide
inhibition of the recombinant FAD domain
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.1.1
additional information
-
additional information
Km-values of the the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli
Spinacia oleracea
1.7.1.1
0.017
-
NADH
recombinant FAD domain
Spinacia oleracea
1.7.1.1
0.034
-
ferricyanide
recombinant FAD domain
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.1.1
additional information
-
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli show a MW of 30000 Da by SDS-PAGE
Spinacia oleracea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.1.1
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli
Spinacia oleracea
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.1
additional information
-
-
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.1.1
additional information
the isolated flavin-containing domain is capable of reducing cytochrome b5 directly
392916
Spinacia oleracea
?
-
-
-
-
1.7.1.1
nitrate + NADH
-
392916
Spinacia oleracea
nitrite + NAD+ + H2O
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.1.1
6.5
-
-
Spinacia oleracea