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Literature summary extracted from

  • Jones, P.W.; Mhuimhneachain, M.N.
    The activity and stability of wheat nitrate reductase in vitro (1985), Phytochemistry, 24, 385-392.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.7.1.1 the decay rate of nitrate reductase activity in crude extracts is due to spontaneous dissociation of the enzyme and to the effects of two decay factors, one present in the 0-30% and the other in the 50-70% saturated (NH4)2SO4 fraction of the crude extract Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.1 202000
-
 sucrose density gradient centrifugation, gel filtration Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.1 seedling
-
 Triticum aestivum
-
1.7.1.1 shoot
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 21.8
-
-
 Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 nitrate + NADH
-
 Triticum aestivum nitrite + NAD+ + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.1 30
-
 30-50% (NH4)2SO4, 150 mM potassium phosphate buffer, pH 7.5, 0.01 mM FAD, 1 mM dithiothreitol, half-life: 36.8 min Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Triticum aestivum