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Literature summary extracted from

  • Sherrard, J.H.; Dalling, M.J.
    In vitro stability of nitrate reductase from wheat leaves (1979), Plant Physiol., 63, 346-353.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.7.1.1 FAD stabilizes at 25°C, at 10°C FAD has no effect on stability Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.033
-
 NADH
-
 Triticum aestivum
1.7.1.1 0.34
-
 nitrate
-
 Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Triticum aestivum
-
 L. cv. Olympic
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.1 leaf from 8-day-old seedlings Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 23.1
-
-
 Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 nitrate + NADH
-
 Triticum aestivum nitrite + NAD+ + H2O
-
 ?
1.7.1.1 reduced methyl viologen + nitrate
-
 Triticum aestivum methyl viologen + nitrite
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.1 10
-
 half-life: 94 min Triticum aestivum
1.7.1.1 25
-
 half-life is 28 min, half-life in presence of GAD: 70 min Triticum aestivum
1.7.1.1 30
-
 transition temperature Triticum aestivum
1.7.1.1 40
-
 t1/2: 1 min Triticum aestivum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.1 7.5
-
 highest stability, unstable below Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Triticum aestivum