BRENDA - Enzyme Database show

Purification and characterization of NADH dehydrogenase from Bacillus subtilis

Bergsma, J.; van Dongen, M.B.M.; Konings, W.N.; Eur. J. Biochem. 128, 151-157 (1982)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.6.99.3
adenosine
slight inhibition
Bacillus subtilis
1.6.99.3
deoxyadenosine
slight inhibition
Bacillus subtilis
1.6.99.3
nicotinic acid amide
slight inhibition
Bacillus subtilis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.6.99.3
membrane
-
Bacillus subtilis
16020
-
1.6.99.3
vesicular fraction
-
Bacillus subtilis
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.6.99.3
64000
-
x * 64000, SDS-PAGE
Bacillus subtilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.6.99.3
Bacillus subtilis
-
W23
-
1.6.99.3
Bacillus subtilis W23
-
W23
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.6.99.3
24.67
-
-
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.6.99.3
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
-
392820
Bacillus subtilis
NAD+ + ?
-
-
-
?
1.6.99.3
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
-
392820
Bacillus subtilis W23
NAD+ + ?
-
-
-
?
1.6.99.3
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
less than 6% of the activity with NADH
392820
Bacillus subtilis
NADP+ + ?
-
-
-
?
1.6.99.3
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
less than 6% of the activity with NADH
392820
Bacillus subtilis W23
NADP+ + ?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.6.99.3
?
x * 64000, SDS-PAGE
Bacillus subtilis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.6.99.3
37
-
activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.6.99.3
7.5
8
activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.6.99.3
FAD
contains 1 molecule FAD per molecule
Bacillus subtilis
1.6.99.3
NADH
-
Bacillus subtilis
1.6.99.3
NADPH
less than 6% of the activity with NADH in reaction with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.6.99.3
FAD
contains 1 molecule FAD per molecule
Bacillus subtilis
1.6.99.3
NADH
-
Bacillus subtilis
1.6.99.3
NADPH
less than 6% of the activity with NADH in reaction with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.6.99.3
adenosine
slight inhibition
Bacillus subtilis
1.6.99.3
deoxyadenosine
slight inhibition
Bacillus subtilis
1.6.99.3
nicotinic acid amide
slight inhibition
Bacillus subtilis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.6.99.3
membrane
-
Bacillus subtilis
16020
-
1.6.99.3
vesicular fraction
-
Bacillus subtilis
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.6.99.3
64000
-
x * 64000, SDS-PAGE
Bacillus subtilis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.6.99.3
24.67
-
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.6.99.3
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
-
392820
Bacillus subtilis
NAD+ + ?
-
-
-
?
1.6.99.3
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
-
392820
Bacillus subtilis W23
NAD+ + ?
-
-
-
?
1.6.99.3
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
less than 6% of the activity with NADH
392820
Bacillus subtilis
NADP+ + ?
-
-
-
?
1.6.99.3
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
less than 6% of the activity with NADH
392820
Bacillus subtilis W23
NADP+ + ?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.6.99.3
?
x * 64000, SDS-PAGE
Bacillus subtilis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.6.99.3
37
-
activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.6.99.3
7.5
8
activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis