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Literature summary extracted from

  • Iobbi-Nivol, C.; Pommier, J.; Simala-Grant, J.; Mejean, V.; Giordano, G.
    High substrate specificity and induction characteristics of trimethylamine N-oxide reductase of Escherichia coli (1996), Biochim. Biophys. Acta, 1294, 77-82.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.3 0.02
-
 4-methylmorpholine N-oxide
-
 Escherichia coli
1.7.2.3 0.07
-
 Trimethylamine N-oxide
-
 Escherichia coli
1.7.2.3 0.26
-
 alpha-picoline N-oxide
-
 Escherichia coli
1.7.2.3 0.32
-
 gamma-picoline N-oxide
-
 Escherichia coli
1.7.2.3 0.75
-
 3-hydroxypyridine N-oxide
-
 Escherichia coli
1.7.2.3 1.14
-
 N,N-dimethylhexylamine N-oxide
-
 Escherichia coli
1.7.2.3 1.25
-
 N,N-dimethyldodecylamine N-oxide
-
 Escherichia coli
1.7.2.3 1.86
-
 Pyridine N-oxide
-
 Escherichia coli
1.7.2.3 2.01
-
 2-chloropyridine N-oxide
-
 Escherichia coli
1.7.2.3 2.15
-
 4-chloropyridine N-oxide
-
 Escherichia coli
1.7.2.3 4.74
-
 N-cyclohexyldimethylamine N-oxide
-
 Escherichia coli
1.7.2.3 5.43
-
 3-picoline N-oxide
-
 Escherichia coli
1.7.2.3 7.39
-
 hydroxylamine N-oxide
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.3 Escherichia coli
-
 inducible enzyme
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.3 33
-
 purified enzyme, assay performed in 100 mM phosphate buffer, pH 6.8 with a trimethylamine N-oxide concentration 0.5 mM and a concentration 20 mM of 2-hydroxypyridine N-oxide Escherichia coli
1.7.2.3 34
-
 purified enzyme, assay performed in 100 mM phosphate buffer, pH 6.8 with a trimethylamine N-oxide concentration 0.5 mM Escherichia coli
1.7.2.3 35
-
 purified enzyme, assay performed in 100 mM phosphate buffer, pH 6.8 with a trimethylamine N-oxide concentration 0.5 mM and a concentration 20 mM of tetramethylenesulfoxide Escherichia coli
1.7.2.3 36
-
 purified enzyme, assay performed in 100 mM phosphate buffer, pH 6.8 with a trimethylamine N-oxide concentration 0.5 mM and a concentration 20 mM of picolinic acid N-oxide or a concentration 20 mM of dimethylsulfoxide Escherichia coli
1.7.2.3 105
-
 purified enzyme, with trimethylamine N-oxide as substrate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.3 2-chloropyridine N-oxide + electron donor
-
 Escherichia coli 2-chloropyridine + oxidized electron donor + H2O
-
 ?
1.7.2.3 3-hydroxypyridine N-oxide + electron donor
-
 Escherichia coli 3-hydroxypyridine + oxidized electron donor + H2O
-
 ?
1.7.2.3 3-picoline N-oxide + electron donor
-
 Escherichia coli 3-picoline + oxidized electron donor + H2O
-
 ?
1.7.2.3 4-chloropyridine N-oxide + electron donor
-
 Escherichia coli 4-chloropyridine + oxidized electron donor + H2O
-
 ?
1.7.2.3 4-methylmorpholine N-oxide + electron donor good substrate Escherichia coli 4-methylmorpholine + oxidized electron donor + H2O
-
 ?
1.7.2.3 alpha-picoline N-oxide + electron donor
-
 Escherichia coli alpha-picoline + H2O + oxidized electron donor
-
 ?
1.7.2.3 gamma-picoline N-oxide + electron donor
-
 Escherichia coli gamma-picoline + H2O + oxidized electron donor
-
 ?
1.7.2.3 hydroxylamine N-oxide + electron donor
-
 Escherichia coli hydroxylamine + H2O + oxidized electron donor
-
 ?
1.7.2.3 N,N-dimethyldodecylamine N-oxide + electron donor
-
 Escherichia coli N,N-dimethyldodecylamine + H2O + oxidized electron donor
-
 ?
1.7.2.3 N,N-dimethylhexylamine N-oxide + electron donor
-
 Escherichia coli N,N-dimethylhexylamine + oxidized electron donor + H2O
-
 ?
1.7.2.3 N-cyclohexyldimethylamine N-oxide + electron donor
-
 Escherichia coli N-cyclohexyldimethylamine + oxidized electron donor + H2O
-
 ?
1.7.2.3 pyridine N-oxide + electron donor
-
 Escherichia coli pyridine + oxidized electron donor + H2O
-
 ?
1.7.2.3 trimethylamine N-oxide + electron donor benzyl viologen as electron donor Escherichia coli trimethylamine + oxidized electron donor + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.3 13.8
-
 N,N-dimethylhexylamine N-oxide
-
 Escherichia coli
1.7.2.3 45.6
-
 N-cyclohexyldimethylamine N-oxide
-
 Escherichia coli
1.7.2.3 49.2
-
 3-hydroxypyridine N-oxide
-
 Escherichia coli
1.7.2.3 49.6
-
 gamma-picoline N-oxide
-
 Escherichia coli
1.7.2.3 53.5
-
 Pyridine N-oxide
-
 Escherichia coli
1.7.2.3 66.7
-
 4-chloropyridine N-oxide
-
 Escherichia coli
1.7.2.3 70
-
 hydroxylamine N-oxide
-
 Escherichia coli
1.7.2.3 96.5
-
 4-methylmorpholine N-oxide
-
 Escherichia coli
1.7.2.3 101
-
 2-chloropyridine N-oxide
-
 Escherichia coli
1.7.2.3 102
-
 alpha-picoline N-oxide
-
 Escherichia coli
1.7.2.3 137
-
 3-picoline N-oxide
-
 Escherichia coli
1.7.2.3 151
-
 Trimethylamine N-oxide
-
 Escherichia coli
1.7.2.3 155
-
 N,N-dimethyldodecylamine N-oxide
-
 Escherichia coli