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Literature summary extracted from

  • Silvestro, A.; Pommier, J.; Giordano, G.
    The inducible trimethylamine-N-oxide reductase of Escherichia coli K12: biochemical and immunological studies (1988), Biochim. Biophys. Acta, 954, 1-13.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.3 Cu2+ 52% inhibition at 0.1 mM Escherichia coli
1.7.2.3 EGTA 38% inhibition at 10 mM Escherichia coli
1.7.2.3 KCN 20% inhibition at 1 mM Escherichia coli
1.7.2.3 NaN3 slight Escherichia coli
1.7.2.3 p-chloromercuribenzoate slight Escherichia coli
1.7.2.3 Urea 50% inhibition at 6 mM Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.3 Iron 50% stimulation by 0.1 mM Fe3+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.3 110000
-
 a single inducible trimethylamine-N-oxide reductase which can exist as a dimer, MW: 230000, or a monomer, MW: 110000, gel filtration Escherichia coli
1.7.2.3 230000
-
 a single inducible trimethylamine-N-oxide reductase which can exist as a dimer, MW: 230000 or a monomer, MW: 110000, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.3 Escherichia coli
-
 inducible enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.3 using heat treatment, column chromatography on DEAE-cellulose, Sephadex G-200, DEAE-Sepharose CL-6B and hydroxyapatite Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.3 additional information
-
-
 Escherichia coli
1.7.2.3 237
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.7.2.3 -80°C, 45% loss of activity after 30 days Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.3 adenosine N-oxide + electron donor
-
 Escherichia coli adenosine + H2O + oxidized electron donor
-
 ?
1.7.2.3 chlorate + electron donor
-
 Escherichia coli ?
-
 ?
1.7.2.3 hydroxylamine N-oxide + electron donor
-
 Escherichia coli hydroxylamine + H2O + oxidized electron donor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.3 dimer a single inducible trimethylamine-N-oxide reductase which can exist as a dimer, MW 230000 or a monomer, MW 110000 Escherichia coli
1.7.2.3 monomer a single inducible trimethylamine-N-oxide reductase which can exist as a dimer, MW 230000 or a monomer, MW 110000 Escherichia coli