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Literature summary extracted from

  • Violet, M.; Medani, C.L.; Giordano, G.
    Trimethylamine N-oxide (TMAO) reductases from Escherichia coli K-12 (1985), FEMS Microbiol. Lett., 27, 85-91.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.3 KCN 10-15% inhibition of the inducible and of the constitutive enzyme at 10 mM Escherichia coli
1.7.2.3 N-ethylmaleimide both inducible and constitutive enzyme are affected equally at 5 mM Escherichia coli
1.7.2.3 NaN3 5% inhibition of inducible enzyme and 50-60% inhibition of constitutive enzyme at 10 mM Escherichia coli
1.7.2.3 p-chloromercuribenzoate 2% inhibition of inducible enzyme and 50-60% inhibition of constitutive enzyme at 5 mM Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.3 membrane constitutive enzyme Escherichia coli 16020
-
1.7.2.3 soluble inducible enzyme Escherichia coli
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.3 Escherichia coli
-
 a constitutive enzyme and an inducible enzyme with different properties
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.7.2.3 aeration for 10 min causes 10% inactivation Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.7.2.3 -80°C, inducible and constitutive enzyme, 10% loss of activity after 15 days Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.3 trimethylamine N-oxide + electron donor benzyl viologen as electron donor Escherichia coli trimethylamine + oxidized electron donor + H2O
-
 ?
1.7.2.3 trimethylamine N-oxide + electron donor NADH as electron donor Escherichia coli trimethylamine + oxidized electron donor + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.2.3 60
-
 20 min, 10% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.3 5.5
-
 for inducible and constitutive enzyme Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.3 NADH
-
 Escherichia coli