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Literature summary extracted from

  • Yu, S.W.; Kim, Y.R.; Kang, S.O.
    Spectral characterization and chemical modification of FMN-containing ascorbyl free-radical reductase from Pleurotus ostreatus (1999), Biochem. J., 341, 755-763.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.6.5.4 freezing without glycerol, complete loss of activity Pleurotus ostreatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.4 1,10-phenanthroline 10 mM, 16% inhibition Pleurotus ostreatus
1.6.5.4 2,2'-dipyridyl 10 mM, 21% inhibition Pleurotus ostreatus
1.6.5.4 2,4,6-Trinitrobenzenesulfonic acid 1 mM, 54% inhibition Pleurotus ostreatus
1.6.5.4 5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, 23% inhibition Pleurotus ostreatus
1.6.5.4 8-hydroxyquinoline 10 mM, 15% inhibition Pleurotus ostreatus
1.6.5.4 Cu2+ 0.5 mM, complete inhibition Pleurotus ostreatus
1.6.5.4 diacetyl 1 mM, 55% inhibition Pleurotus ostreatus
1.6.5.4 diethyl dicarbonate 0.1 mM, 62% inhibition Pleurotus ostreatus
1.6.5.4 EDTA 10 mM, 11% inhibition Pleurotus ostreatus
1.6.5.4 Hg2+ 0.5 mM, complete inhibition Pleurotus ostreatus
1.6.5.4 iodoacetamide 5 mM, 32% inhibition Pleurotus ostreatus
1.6.5.4 NaN3 16 mM, 16% inhibition Pleurotus ostreatus
1.6.5.4 NEM 1 mM, 82% inhibition Pleurotus ostreatus
1.6.5.4 p-chloromercuribenzoate 0.1 mM, complete inhibition Pleurotus ostreatus
1.6.5.4 phenyl mercuric acetate 0.1 mM, 85% inhibition Pleurotus ostreatus
1.6.5.4 Phenylglyoxal 100 mM, 46% inhibition Pleurotus ostreatus
1.6.5.4 pyridoxal 5'-phosphate 1 mM, 46% inhibition Pleurotus ostreatus
1.6.5.4 Trinitromethane 10 mM, 11% inhibition Pleurotus ostreatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.4 0.0021
-
 monodehydroascorbate
-
 Pleurotus ostreatus
1.6.5.4 0.035
-
 NADH
-
 Pleurotus ostreatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.4 cytosol
-
 Pleurotus ostreatus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.4 62000
-
 2 * 62000, SDS-PAGE Pleurotus ostreatus
1.6.5.4 127000
-
 gel filtration Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Pleurotus ostreatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.4
-
 Pleurotus ostreatus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.5.4 NADH + H+ + 2 monodehydroascorbate = NAD+ + 2 ascorbate ping pong mechanism Pleurotus ostreatus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.4 mycelium
-
 Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.4 11.09
-
-
 Pleurotus ostreatus

Storage Stability

EC Number Storage Stability Organism
1.6.5.4 -20°C, 10 mM Tris/HCl buffer, pH 7.4, 2 mM dithiothreitol, 25% glycerol, 1 month, stable Pleurotus ostreatus
1.6.5.4 4°C, 4°C, in absence of dithiothreitol, complete loss of activity within 1 day Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 NADH + 1,4-benzoquinone
-
 Pleurotus ostreatus NAD+ + ?
-
 ?
1.6.5.4 NADH + 2,6-dichlorophenol indophenol
-
 Pleurotus ostreatus NAD+ + ?
-
 ?
1.6.5.4 NADH + monodehydroascorbate
-
 Pleurotus ostreatus NAD+ + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.4 dimer 2 * 62000, SDS-PAGE Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.4 7 9
-
 Pleurotus ostreatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.6.5.4 7 11 stable Pleurotus ostreatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 FMN contains 1 mol of FMN per subunit, flavin is non-covalently bound Pleurotus ostreatus
1.6.5.4 NADH
-
 Pleurotus ostreatus