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Literature summary extracted from
- Purification and characterization of the recombinant Na+-translocating NADH:quinone oxidoreductase from Vibrio cholera (2002), Biochemistry, 41, 3781-3789.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.2.1.1 | cloned under the regulation of the P(BAD) promoter, successfully expressed in Vibrio cholerae | Vibrio cholerae serotype O1 |
| 7.2.1.1 | cloned under the regulation of the PBAD promoter and expressed in Vibrio cholera | Vibrio cholerae serotype O1 |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.1.1 | membrane | associated to | Vibrio cholerae serotype O1 | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.1.1 | Na+ | recombinant enzyme has high specific activity in presence of Na+ | Vibrio cholerae serotype O1 |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.2.1.1 | 360000 | - |
gel filtration | Vibrio cholerae serotype O1 |
| 7.2.1.1 | 360000 | - |
it is likely that the protein is a monomer, calculated molecular mass of 215000 Da with bound detergent, gel filtration | Vibrio cholerae serotype O1 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.1.1 | NADH + ubiquinone | Vibrio cholerae serotype O1 | Na+-translocation | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.1.1 | Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.2.1.1 | - |
Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.1.1 | additional information | the NADH oxidation is coupled to the pumping of Na+ across the membrane | Vibrio cholerae serotype O1 | ? | - |
? | |
| 7.2.1.1 | NADH + ubiquinone | Na+-translocation | Vibrio cholerae serotype O1 | NAD+ + ubiquinol | - |
? | |
| 7.2.1.1 | NADH + ubiquinone | Na+-translocation | Vibrio cholerae serotype O1 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.2.1.1 | Na(+)-translocating NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
| 7.2.1.1 | Na+-NQR | - |
Vibrio cholerae serotype O1 |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.1.1 | FAD | enzyme contains one molecule of noncovalently bound FAD | Vibrio cholerae serotype O1 | |
| 7.2.1.1 | FAD | contains one molecule of covalently bound FAD, believed to be bound to subunit F | Vibrio cholerae serotype O1 | |
| 7.2.1.1 | FMN | enzyme contains 2 molecules of covalently bound FMN | Vibrio cholerae serotype O1 | |
| 7.2.1.1 | FMN | the enzyme contains two molecules of covalently bound FMN, covalently bound to subunits NqrB and NqrC | Vibrio cholerae serotype O1 | |
| 7.2.1.1 | NADH | - |
Vibrio cholerae serotype O1 | |
| 7.2.1.1 | ubiquinone | enzyme contains approximately one bound ubiquinone | Vibrio cholerae serotype O1 | |
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Release 2023.1 (January 2023)
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