Any feedback?
Literature summary extracted from
- A change in ionization of the NADP(H)-binding component (dIII) if proton-translocating transhydrogenase regulates both hydride transfer and nucleotide release (2001), Eur. J. Biochem., 268, 1430-1438.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.1.1.1 | expressed in Escherichia coli | Rhodospirillum rubrum |
| 7.1.1.1 | expression of domain I and of E155W and Y171W mutant domain III | Rhodospirillum rubrum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.1.1.1 | E155W | mutation in domain III, similar catalytic activities as wild-type, used for tryptophan fluorescence measurements | Rhodospirillum rubrum |
| 7.1.1.1 | E155W | mutant of the NADP(H)-binding component | Rhodospirillum rubrum |
| 7.1.1.1 | Y171W | mutation in domain III, similar catalytic activities as wild-type, used for tryptophan fluorescence measurements | Rhodospirillum rubrum |
| 7.1.1.1 | Y171W | the mutant shows wild type activity | Rhodospirillum rubrum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.1 | NADPH + NAD+ + H+[side 1] | Rhodospirillum rubrum | - |
NADP+ + NADH + H+[side 2] | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.1 | Rhodospirillum rubrum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.1.1 | - |
Rhodospirillum rubrum |
| 7.1.1.1 | recombinant domain I and recombinant E155W and Y171W mutant domain III | Rhodospirillum rubrum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.1 | NADPH + NAD+ + H+[side 1] | - |
Rhodospirillum rubrum | NADP+ + NADH + H+[side 2] | - |
? | |
| 7.1.1.1 | NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Rhodospirillum rubrum | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.1.1 | proton-translocating transhydrogenase | - |
Rhodospirillum rubrum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.1 | NAD+ | - |
Rhodospirillum rubrum | |
| 7.1.1.1 | NADH | - |
Rhodospirillum rubrum | |
| 7.1.1.1 | NADP+ | - |
Rhodospirillum rubrum | |
| 7.1.1.1 | NADPH | - |
Rhodospirillum rubrum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
