EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.1.2 | expression of domain I Y235N and Y235F mutants in Escherichia coli | Rhodospirillum rubrum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.6.1.2 | Y235F | mutation resides in the soluble NAD(H)-binding peripheral membrane subunit, i.e. domain I, reconstitution of depleted membranes with mutant domain I gives 44% of activity that is obtained with wild-type domain I reconstituted membranes | Rhodospirillum rubrum |
1.6.1.2 | Y235N | mutation resides in the soluble NAD(H)-binding peripheral membrane subunit, i.e. domain I, reconstitution of depleted membranes with mutant domain I gives 18% of activity that is obtained with wild-type domain I reconstituted membranes | Rhodospirillum rubrum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.1.2 | 0.015 | - |
NADPH | Y235N and Y235F mutants of domain I | Rhodospirillum rubrum | |
1.6.1.2 | 0.03 | - |
NADPH | wild-type domain I | Rhodospirillum rubrum | |
1.6.1.2 | 0.06 | - |
oxidized 3-acetylpyridine adenine dinucleotide | wild-type domain I | Rhodospirillum rubrum | |
1.6.1.2 | 0.6 | - |
oxidized 3-acetylpyridine adenine dinucleotide | Y235F mutant of domain I | Rhodospirillum rubrum | |
1.6.1.2 | 0.8 | - |
oxidized 3-acetylpyridine adenine dinucleotide | Y235N mutant of domain I | Rhodospirillum rubrum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.1.2 | Rhodospirillum rubrum | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.1.2 | NADPH + oxidized 3-acetylpyridine adenine dinucleotide | - |
Rhodospirillum rubrum | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
r |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.1.2 | NAD+ | - |
Rhodospirillum rubrum | |
1.6.1.2 | NADH | - |
Rhodospirillum rubrum | |
1.6.1.2 | NADP+ | - |
Rhodospirillum rubrum | |
1.6.1.2 | NADPH | - |
Rhodospirillum rubrum |