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Literature summary extracted from

  • Diggle, C.; Bizouarn, T.; Cotton, N.P.; Jackson, J.B.
    Properties of the purified, recombinant, NADP(H)-binding domain III of the proton-translocating nicotinamide nucleotide transhydrogenase from Rhodospirillum rubrum (1996), Eur. J. Biochem., 241, 162-170.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.1.2 expression of domains I and III in Escherichia coli Rhodospirillum rubrum

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.2 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.1.2 recombinant domains I and III Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.2 NADH + oxidized 3-acetylpyridine adenine dinucleotide
-
 Rhodospirillum rubrum NAD+ + reduced 3-acetylpyridine adenine dinucleotide
-
 r
1.6.1.2 NADPH + oxidized 3-acetylpyridine adenine dinucleotide catalyzed by a mixture of purified recombinant domains I and III Rhodospirillum rubrum NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.1.2 0.02
-
 oxidized 3-acetylpyridine adenine dinucleotide kcat for recombinant domain III in the presence of saturating amounts of domain I, reduction of acetylpyridine adenine dinucleotide by NADPH Rhodospirillum rubrum
1.6.1.2 1.44
-
 oxidized 3-acetylpyridine adenine dinucleotide kcat for recombinant domain I in the presence of saturating amounts of domain III, reduction of acetylpyridine adenine dinucleotide by NADPH Rhodospirillum rubrum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.2 NAD+
-
 Rhodospirillum rubrum
1.6.1.2 NADH
-
 Rhodospirillum rubrum
1.6.1.2 NADP+
-
 Rhodospirillum rubrum
1.6.1.2 NADPH
-
 Rhodospirillum rubrum