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Literature summary extracted from

  • Fristedt, U.; Rydström, J.; Persson, B.
    Evidence for a nicotineamide nucleotide transhydrogenase in Klebsiella pneumoniae (1994), Biochem. Biophys. Res. Commun., 198, 928-932.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.1.2 palmitoyl-CoA
-
 Escherichia coli
1.6.1.2 palmitoyl-CoA competitive vs. NADPH Klebsiella pneumoniae
1.6.1.2 Phenylarsine oxide 0.48 mM, 50% inhibition of acetylpyridine adenine dinucleotide reduction in inside-out membrane vesicles after 5 min Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.1.2 Li+ not clear whether this reflects a general salt effect or a Li+ specific effect Klebsiella pneumoniae
1.6.1.2 Na+ not clear whether this reflects a general salt effect or a Na+ specific effect Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.1.2 54000
-
 x * 54000, immunoblot with antibodies against beef heart enzyme Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.2 Escherichia coli
-
-
-
1.6.1.2 Klebsiella pneumoniae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.1.2 0.26
-
 activity in inside-out membrane vesicles at pH 7.4, presence of an uncoupler i.e. carbonylcyanide-m-chlorophylhydrazone results in 2fold stimulation Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.2 NADH + NADP+
-
 Escherichia coli NAD+ + NADPH
-
 ?
1.6.1.2 NADH + NADP+
-
 Klebsiella pneumoniae NAD+ + NADPH
-
 r
1.6.1.2 NADPH + oxidized 3-acetylpyridine adenine dinucleotide
-
 Escherichia coli NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
 r
1.6.1.2 NADPH + oxidized 3-acetylpyridine adenine dinucleotide
-
 Klebsiella pneumoniae NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.1.2 ? x * 54000, immunoblot with antibodies against beef heart enzyme Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.1.2 7 8 reduction of oxidized acetylpyridine adenine dinucleotide by NADPH in inside-out membrane vesicles Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.2 NAD+
-
 Escherichia coli
1.6.1.2 NAD+
-
 Klebsiella pneumoniae
1.6.1.2 NADH
-
 Escherichia coli
1.6.1.2 NADH
-
 Klebsiella pneumoniae
1.6.1.2 NADP+
-
 Escherichia coli
1.6.1.2 NADP+
-
 Klebsiella pneumoniae
1.6.1.2 NADPH
-
 Escherichia coli
1.6.1.2 NADPH
-
 Klebsiella pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.1.2 0.00125
-
 palmitoyl-CoA
-
 Klebsiella pneumoniae
1.6.1.2 0.007
-
 palmitoyl-CoA
-
 Escherichia coli