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Literature summary extracted from

  • O'Neal, S.G.; Earle, S.R.; Fisher, R.R.
    The effect of metal ions on mitochondrial pyridine dinucleotide transhydrogenase (1980), Biochim. Biophys. Acta, 589, 217-230.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.1.2 cations prevent from tryptic inactivation, 95% protection with Mn2+, 89% with Ca2+ and 79% with Mg2+ Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.1.2 2,2'-dithiodipyridine 0.2 mM, 45% inhibition Bos taurus
1.6.1.2 2,2'-thiodiethanethiole 0.5 mM, 37% inhibition Bos taurus
1.6.1.2 5,5'-dithiobis(2-nitrobenzoate) 0.02 mM, 51% inhibition, 92% inhibition in the presence of 2 mM Mg2+ Bos taurus
1.6.1.2 Ca2+ 20 mM, 50% inhibition at pH 7.0, maximal inhibition at pH 9.0 Bos taurus
1.6.1.2 formamide disulfide dihydrochloride 0.2 mM, 43% inhibition Bos taurus
1.6.1.2 K+ 200 mM, 50% inhibition at pH 7.9, 300 mM, 40% inhibition at pH 5.5, 95% at pH 8.5 Bos taurus
1.6.1.2 La3+ 0.1 mM, 50% inhibition at pH 7.0, maximal inhibition at pH 8.0 Bos taurus
1.6.1.2 Mg2+ 20 mM, 50% inhibition at pH 7.0, competitive vs. NADPH and thio-NADP+, noncompetitive vs. acetylpyridine adenine dinucleotide and NADH Bos taurus
1.6.1.2 Mn2+ 10 mM, 50% inhibition at pH 7.0, maximal inhibition at pH 9.0 Bos taurus
1.6.1.2 Na+ 200 mM, 50% inhibition at pH 7.9, 300 mM, 40% inhibition at pH 5.5, 95% at pH 8.5 Bos taurus
1.6.1.2 p-chloromercuribenzoate 0.003 mM, 25% inhibition Bos taurus
1.6.1.2 Sr2+ 25 mM, 50% inhibition at pH 7.0 Bos taurus
1.6.1.2 Tl+ 20 mM, 50% inhibition at pH 7.9 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.1.2 mitochondrial membrane
-
 Bos taurus 31966
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.1.2 heart
-
 Bos taurus
-

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.1.2 additional information
-
 protection against thermal inactivation by cations, half-maximal protection is afforded at: 1-2 mM Mg2+, 2 mM Ca2+, 0.5 mM Mn2+ and 0.025 mM La3+ Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.2 NAD+
-
 Bos taurus
1.6.1.2 NADH
-
 Bos taurus
1.6.1.2 NADP+
-
 Bos taurus
1.6.1.2 NADPH
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.1.2 2.5
-
 Mg2+ at pH 7.0 Bos taurus