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Literature summary extracted from

  • Clarke, D.M.; Bragg, P.D.
    Cloning and expression of the transhydrogenase gene of Escherichia coli (1985), J. Bacteriol., 162, 367-373.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.1
-
 Escherichia coli
7.1.1.1 expressed in Escherichia coli JM83 cells Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.1 membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.1 47000
-
 1 * 50000 + 1 * 47000, SDS-PAGE Escherichia coli
7.1.1.1 50000
-
 1 * 50000 + 1 * 47000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+[side 1] Escherichia coli
-
 NADP+ + NADH + H+[side 2]
-
 r
7.1.1.1 NADPH + NAD+ + H+[side 1] Escherichia coli MV-12
-
 NADP+ + NADH + H+[side 2]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.1 Escherichia coli
-
-
-
7.1.1.1 Escherichia coli MV-12
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+[side 1]
-
 Escherichia coli NADP+ + NADH + H+[side 2]
-
 r
7.1.1.1 NADPH + NAD+ + H+[side 1]
-
 Escherichia coli MV-12 NADP+ + NADH + H+[side 2]
-
 r
7.1.1.1 NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
 Escherichia coli NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
 r
7.1.1.1 NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
 Escherichia coli MV-12 NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
 r

Subunits

EC Number Subunits Comment Organism
7.1.1.1 heterodimer 1 * 50000 + 1 * 47000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
7.1.1.1 pyridine nucleotide transhydrogenase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.1 NAD+
-
 Escherichia coli
7.1.1.1 NADH
-
 Escherichia coli
7.1.1.1 NADP+
-
 Escherichia coli
7.1.1.1 NADPH
-
 Escherichia coli