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Literature summary extracted from

  • Cotton, N.P.J.; Lever, T.M.; Nore, B.F.; Jones, M.R.; Jackson, J.B.
    The coupling between protonmotive force and the NAD(P)+ transhydrogenase in chromatophores from photosynthetic bacteria [published erratum appears in Eur J Biochem 1989 Oct 1;184(3):729] (1989), Eur. J. Biochem., 182, 593-603.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.1.1 light forward reaction in chromatophores is accelerated more than 20fold during illumination with photosynthetically active light Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.1 0.009
-
thio-NADP+ pH 6.3, in the light Rhodobacter capsulatus
7.1.1.1 0.013
-
thio-NADP+ pH 7.6, in the light Rhodobacter capsulatus
7.1.1.1 0.019
-
thio-NADP+ pH 7.6, in the dark Rhodobacter capsulatus
7.1.1.1 0.02
-
NADH pH 6.3, in the light Rhodobacter capsulatus
7.1.1.1 0.022
-
NADH pH 6.3, in the dark Rhodobacter capsulatus
7.1.1.1 0.025
-
NADH pH 7.6, in the light Rhodobacter capsulatus
7.1.1.1 0.029
-
thio-NADP+ pH 9.0, in the light Rhodobacter capsulatus
7.1.1.1 0.032
-
thio-NADP+ pH 6.3, in the dark Rhodobacter capsulatus
7.1.1.1 0.033
-
NADH pH 9.0, in the light Rhodobacter capsulatus
7.1.1.1 0.045
-
thio-NADP+ pH 9.0, in the dark Rhodobacter capsulatus
7.1.1.1 0.055
-
thio-NADP+ pH 10.0, in the light Rhodobacter capsulatus
7.1.1.1 0.064
-
NADH pH 7.6, in the dark Rhodobacter capsulatus
7.1.1.1 0.066
-
NADH pH 9.0, in the dark Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.1 Ca2+ required for catalytic activity Rhodobacter capsulatus
7.1.1.1 Ca2+ strong activation of forward reaction in the light at concentrations above 0.1 mM Rhodobacter capsulatus
7.1.1.1 K+ strong activation of forward reaction, i.e. the reduction of NADP+ by NADH, in the light at concentrations of approx. 50-80 mM, half-maximal activation at 10 mM Rhodobacter capsulatus
7.1.1.1 Mg2+ required for catalytic activity Rhodobacter capsulatus
7.1.1.1 Mg2+ strong activation of forward reaction, i.e. the reduction of NADP+ by NADH, in the light at concentrations above 0.1 mM Rhodobacter capsulatus
7.1.1.1 Na+ strong activation of forward reaction, i.e. the reduction of NADP+ by NADH, in the light at concentrations of approx. 50-80 mM, half-maximal activation at 10 mM Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.1 Rhodobacter capsulatus
-
formerly Rhodopseudomonas capsulata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.1 NADH + thio-NADP+
-
Rhodobacter capsulatus NAD+ + thio-NADPH
-
r

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.1 NAD+
-
Rhodobacter capsulatus
7.1.1.1 NADH
-
Rhodobacter capsulatus
7.1.1.1 NADP+
-
Rhodobacter capsulatus
7.1.1.1 NADPH
-
Rhodobacter capsulatus