BRENDA - Enzyme Database

Purification and properties of the pyridine nucleotide transhydrogenase from Pseudomonas aeruginosa

Cohen, P.T.; Kaplan, N.O.; J. Biol. Chem. 245, 2825-2836 (1970) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.6.1.1
p-hydroxymercuribenzoate
-
Pseudomonas aeruginosa
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.6.1.1
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.6.1.1
-
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.6.1.1
NADP+ + NADH
diaphorase-type reactions with NAD(P)H, K3Fe(CN)6 and 2,6-dichlorophenol indophenol
392590
Pseudomonas aeruginosa
NADPH + NAD+
-
392590
Pseudomonas aeruginosa
?
1.6.1.1
NADPH + 3-acetylpyridine-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + 3-acetylpyridine-NADH
-
392590
Pseudomonas aeruginosa
?
1.6.1.1
NADPH + deamino-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + deamino-NADH
-
392590
Pseudomonas aeruginosa
?
1.6.1.1
NADPH + pyridine aldehyde-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + pyridine aldehyde-NADH
-
392590
Pseudomonas aeruginosa
?
1.6.1.1
NADPH + thio-NADP+ + H+[side 1]
-
392590
Pseudomonas aeruginosa
NADP+ + thio-NADPH + H+[side 2]
-
392590
Pseudomonas aeruginosa
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.6.1.1
51
-
25 min, 50% inactivation, accelerated by addition of NADPH, reactivation by FAD
Pseudomonas aeruginosa
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.6.1.1
233
333
NADPH
-
Pseudomonas aeruginosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.6.1.1
p-hydroxymercuribenzoate
-
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.6.1.1
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.6.1.1
NADP+ + NADH
diaphorase-type reactions with NAD(P)H, K3Fe(CN)6 and 2,6-dichlorophenol indophenol
392590
Pseudomonas aeruginosa
NADPH + NAD+
-
392590
Pseudomonas aeruginosa
?
1.6.1.1
NADPH + 3-acetylpyridine-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + 3-acetylpyridine-NADH
-
392590
Pseudomonas aeruginosa
?
1.6.1.1
NADPH + deamino-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + deamino-NADH
-
392590
Pseudomonas aeruginosa
?
1.6.1.1
NADPH + pyridine aldehyde-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + pyridine aldehyde-NADH
-
392590
Pseudomonas aeruginosa
?
1.6.1.1
NADPH + thio-NADP+ + H+[side 1]
-
392590
Pseudomonas aeruginosa
NADP+ + thio-NADPH + H+[side 2]
-
392590
Pseudomonas aeruginosa
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.6.1.1
51
-
25 min, 50% inactivation, accelerated by addition of NADPH, reactivation by FAD
Pseudomonas aeruginosa
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.6.1.1
233
333
NADPH
-
Pseudomonas aeruginosa