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Literature summary extracted from

  • Sakuraba, H.; Takamatsu, Y.; Satomura, T.; Kawakami, R.; Ohshima, T.
    Purification, characterization, and application of a novel dye-linked L-proline dehydrogenase from a hyperthermophilic archaeon, Thermococcus profundus (2001), Appl. Environ. Microbiol., 67, 1470-1475.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.5.2 2
-
 L-proline
-
 Thermococcus profundus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.5.2 40000
-
 alpha,beta, 2 * 50000 + 2 * 40000, SDS-PAGE Thermococcus profundus
1.5.5.2 50000
-
 alpha,beta, 2 * 50000 + 2 * 40000, SDS-PAGE Thermococcus profundus
1.5.5.2 160000
-
 PAGE Thermococcus profundus

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.2 Thermococcus profundus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.5.2
-
 Thermococcus profundus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.2 L-proline + acceptor + H2O
-
 Thermococcus profundus (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.5.2 tetramer alpha,beta, 2 * 50000 + 2 * 40000, SDS-PAGE Thermococcus profundus

Synonyms

EC Number Synonyms Comment Organism
1.5.5.2 dye-linked L-proline dehydrogenase
-
 Thermococcus profundus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.5.2 70
-
 10 min, fully active Thermococcus profundus
1.5.5.2 80
-
 120 min, 40% of activity remains Thermococcus profundus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.5.2 4 10 50°C, 10 min, stable Thermococcus profundus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.5.2 FAD
-
 Thermococcus profundus