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Literature summary extracted from

  • Meile, L.; Leisinger, T.
    Purification and properties of the bifunctional proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase from Pseudomonas aeruginosa (1982), Eur. J. Biochem., 129, 67-75.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.99.B2 Cd2+
-
 Pseudomonas aeruginosa
1.5.99.B2 Hg2+
-
 Pseudomonas aeruginosa
1.5.99.B2 Mg2+ slightly Salmonella enterica subsp. enterica serovar Typhimurium
1.5.99.B2 Zn2+
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.B2 0.018
-
 FAD
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.B2 119000
-
 2 * 119000, SDS-PAGE Pseudomonas aeruginosa
1.5.99.B2 242000
-
 gel filtration, native PAGE leads to two active forms of 24000 and 47000 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.B2 L-proline + acceptor + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?
1.5.99.B2 L-proline + acceptor + H2O Pseudomonas aeruginosa
-
 (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Pseudomonas aeruginosa
-
-
-
1.5.99.B2 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.B2
-
 Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.99.B2 L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol also active as 1-pyrroline-5-carboxylate dehydrogenase Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.B2 2.75
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.5.99.B2 5.26
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.5.99.B2 -20°C, pH 7.6, 50% glycerol, 1 month Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + acceptor + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?
1.5.99.B2 L-proline + acceptor + H2O
-
 Pseudomonas aeruginosa (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?
1.5.99.B2 L-proline + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c Salmonella enterica subsp. enterica serovar Typhimurium (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?
1.5.99.B2 L-proline + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c Pseudomonas aeruginosa (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?
1.5.99.B2 L-proline + acceptor + H2O highly specific for L-form of proline Pseudomonas aeruginosa (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?
1.5.99.B2 L-proline + FAD
-
 Pseudomonas aeruginosa (S)-1-pyrroline-5-carboxylate + FADH2
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.99.B2 dimer 2 * 119000, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.B2 9.5
-
 L-proline + phenazine methosulfate Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD
-
 Pseudomonas aeruginosa