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Literature summary extracted from

  • Frisell, W.R.; Mackenzie, C.G.
    Sarcosine dehydrogenase and dimethylglycine dehydrogenase (rat liver, monkey liver) (1970), Methods Enzymol., 17A, 976-981.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.8.3 Methoxyacetate
-
Macaca mulatta
1.5.8.3 Methoxyacetate
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.3 0.32 1 sarcosine
-
Rattus norvegicus
1.5.8.3 0.32 1 sarcosine
-
Macaca mulatta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.8.3 mitochondrion
-
Rattus norvegicus 5739
-
1.5.8.3 mitochondrion
-
Macaca mulatta 5739
-
1.5.8.4 mitochondrion
-
Rattus norvegicus 5739
-
1.5.8.4 mitochondrion
-
Macaca mulatta 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.8.3 Fe2+
-
Macaca mulatta
1.5.8.3 Fe2+ the enzyme contains 1-2 atoms non-heme iron per 300000 g of protein Rattus norvegicus
1.5.8.4 Nonheme iron
-
Rattus norvegicus
1.5.8.4 Nonheme iron
-
Macaca mulatta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.3 sarcosine + acceptor + H2O Rattus norvegicus acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate glycine + formaldehyde + reduced acceptor
-
?
1.5.8.3 sarcosine + acceptor + H2O Macaca mulatta acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate glycine + formaldehyde + reduced acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.3 Macaca mulatta
-
monkey
-
1.5.8.3 Rattus norvegicus
-
-
-
1.5.8.4 Macaca mulatta
-
monkey
-
1.5.8.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.3
-
Macaca mulatta
1.5.8.4 using ammonium sulfate treatment and dissolution in 7.5 mM potassium phosphate Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.3 liver
-
Rattus norvegicus
-
1.5.8.3 liver
-
Macaca mulatta
-
1.5.8.4 liver
-
Rattus norvegicus
-
1.5.8.4 liver
-
Macaca mulatta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.8.4 129
-
potassium phosphate fraction Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.5.8.3 -4°C, lyophilized, 1 week Rattus norvegicus
1.5.8.3 -4°C, lyophilized, 1 week Macaca mulatta
1.5.8.4 -4°C, lyophilized, 1 week Rattus norvegicus
1.5.8.4 -4°C, lyophilized, 1 week Macaca mulatta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.3 sarcosine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Rattus norvegicus glycine + formaldehyde + reduced acceptor
-
?
1.5.8.3 sarcosine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Macaca mulatta glycine + formaldehyde + reduced acceptor
-
?
1.5.8.4 N,N-dimethylglycine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Rattus norvegicus sarcosine + formaldehyde + reduced acceptor
-
?
1.5.8.4 N,N-dimethylglycine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Macaca mulatta sarcosine + formaldehyde + reduced acceptor
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.8.3 8
-
sarcosine + phenazine methosulfate Rattus norvegicus
1.5.8.3 8
-
sarcosine + phenazine methosulfate Macaca mulatta
1.5.8.4 8.5 9 dimethylglycine + phenazine methosulfate Rattus norvegicus
1.5.8.4 8.5 9 dimethylglycine + phenazine methosulfate Macaca mulatta

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.8.3 1.1
-
Methoxyacetate reversible,competitive Macaca mulatta