Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Porter, D.H.; Cook, R.J.; Wagner, C.
    Enzymatic properties of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver (1985), Arch. Biochem. Biophys., 243, 396-407.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.8.3 Methoxyacetate
-
 Rattus norvegicus
1.5.8.4 Methoxyacetate competitive inhibitor at low substrate concentrations Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.3 0.32 1 sarcosine
-
 Rattus norvegicus
1.5.8.4 0.05
-
 dimethylglycine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.8.3 mitochondrion
-
 Rattus norvegicus 5739
-
1.5.8.4 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.3 sarcosine + acceptor + H2O Rattus norvegicus acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate glycine + formaldehyde + reduced acceptor
-
 ?
1.5.8.4 N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor Rattus norvegicus during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
 ?
1.5.8.4 N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor Rattus norvegicus reaction of the choline degradation pathway sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.3 Rattus norvegicus
-
-
-
1.5.8.4 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.3 liver
-
 Rattus norvegicus
-
1.5.8.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.8.4 0.268
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.3 sarcosine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Rattus norvegicus glycine + formaldehyde + reduced acceptor
-
 ?
1.5.8.4 N,N-dimethylglycine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Rattus norvegicus sarcosine + formaldehyde + reduced acceptor
-
 ?
1.5.8.4 N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate Rattus norvegicus sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
 ?
1.5.8.4 N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor reaction of the choline degradation pathway Rattus norvegicus sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.8.3 0.065
-
 sarcosine
-
 Rattus norvegicus
1.5.8.4 0.14
-
 N,N-dimethylglycine
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.3 FAD covalent bound FAD linked via 8a-position of the isoalloxazin ring to the imidazole N(3) of a histidine residue, structural homology between the amino acid sequence of the flavin-peptides Rattus norvegicus
1.5.8.3 tetrahydrofolate
-
 Rattus norvegicus
1.5.8.4 FAD covalently bound Rattus norvegicus
1.5.8.4 Tetrahydropteroylpentaglutamate
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.8.3 0.26
-
 Methoxyacetate competitive Rattus norvegicus