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Literature summary extracted from

  • Simkovic, M.; Degala, G.D.; Eaton, S.S.; Frerman, F.E.
    Expression of human electron transfer flavoprotein-ubiquinone oxidoreductase from baculovirus vector: kinetic and spectral characterization of the human protein (2002), Biochem. J., 364, 659-667.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.5.1 expression in Sf9 insect cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.5.1 0.00013
-
 electron-transferring flavoprotein
-
 Homo sapiens
1.5.5.1 0.0049
-
 ubiquinone-2
-
 Homo sapiens
1.5.5.1 0.0081
-
 ubiquinone-1
-
 Homo sapiens
1.5.5.1 0.0084
-
 decylubiquinone
-
 Homo sapiens
1.5.5.1 0.0148
-
 ubiquinone-4
-
 Homo sapiens
1.5.5.1 0.0154
-
 semiquinone electron-transferring flavoprotein
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.5.1 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.5.1 64000
-
 x * 64000, assumed to be a monomer in the mitochondrial membrane, immunoblot Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.5.1 recombinant enzyme, DEAE-Sepharose, HA-Ultrogel, Mono Q Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.5.1 17.6
-
 recombinant enzyme, ubiquinone-1 reduction Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.1 reduced electron-transferring flavoprotein + 2,3-dimethoxy-5-methyl-6-(3-methylbut-2-en)-1,4-benzoquinone
-
 Homo sapiens electron-transferring flavoprotein + 2,3-dimethoxy-5-methyl-6-(3-methylbut-2-en)-1,4-benzoquinol
-
 ?
1.5.5.1 reduced electron-transferring flavoprotein + decylubiquinone
-
 Homo sapiens electron-transferring flavoprotein + decylubiquinol
-
 ?
1.5.5.1 reduced electron-transferring flavoprotein + ubiquinone-2
-
 Homo sapiens electron-transferring flavoprotein + ubiquinol-2
-
 ?
1.5.5.1 reduced electron-transferring flavoprotein + ubiquinone-4
-
 Homo sapiens electron-transferring flavoprotein + ubiquinol-4
-
 ?
1.5.5.1 semiquinone electron transferring flavoprotein + ubiquinone Q1
-
 Homo sapiens electron-transferring flavoprotein + hydroquinone electron transferring flavoprotein + ubiquinol Q1
-
 r
1.5.5.1 semiquinone electron-transferring flavoprotein + ubiquinone Q1
-
 Homo sapiens electron-transferring flavoprotein + hydroquinone electron-transferring flavoprotein + ubiquinol Q1
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.5.1 ? x * 64000, assumed to be a monomer in the mitochondrial membrane, immunoblot Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.5.1 21.4
-
 electron-transferring flavoprotein
-
 Homo sapiens
1.5.5.1 33.5
-
 ubiquinone-1
-
 Homo sapiens
1.5.5.1 35.5
-
 ubiquinone-4
-
 Homo sapiens
1.5.5.1 74.3
-
 decylubiquinone
-
 Homo sapiens
1.5.5.1 78.8
-
 ubiqinone-2
-
 Homo sapiens
1.5.5.1 81.4
-
 semiquinone electron-transferring flavoprotein
-
 Homo sapiens