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Literature summary extracted from

  • Naranjo, L.; Martin de Valmaseda, E.; Banuelos, O.; Lopez, P.; Riano, J.; Casqueiro, J.; Martin, J.F.
    Conversion of pipecolic acid into lysine in Penicillium chrysogenum requires pipecolate oxidase and saccharopine reductase: characterization of the lys7 gene encoding saccharopine reductase (2001), J. Bacteriol., 183, 7165-7172.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.10
-
 Penicillium chrysogenum
1.5.3.7 expressed in Escherichia coli DH10B Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.10 48000
-
 predicted from gene sequence Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.7 L-pipecolate + O2 Penicillium chrysogenum
-
 2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2 the product reacts with water to form 2-aminoadipate 6-semialdehyde ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.7 Penicillium chrysogenum
-
-
-
1.5.1.10 Penicillium chrysogenum Q96TW2
-
-
1.5.3.7 Penicillium chrysogenum
-
 strains Wis 54-1255, HS1-, and 10.25
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.7 0.381
-
 crude extract, strain Wis 54-1255 Penicillium chrysogenum
1.5.1.7 0.402
-
 crude extract, strain HS1 Penicillium chrysogenum
1.5.1.7 0.45
-
 crude extract, mutant strain 7.2 Penicillium chrysogenum
1.5.1.7 0.571
-
 crude extract, mutant strain 10.25 Penicillium chrysogenum
1.5.1.10 0.044
-
-
 Penicillium chrysogenum
1.5.3.7 0.002
-
 strain Wis 54-1255, HS1-, 10.25 Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Penicillium chrysogenum L-lysine + 2-oxoglutarate + NADH
-
 ?
1.5.1.10 2-aminoadipate 6-semialdehyde + L-glutamate + NADPH
-
 Penicillium chrysogenum N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.3.7 L-pipecolate + O2
-
 Penicillium chrysogenum 2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2 the product reacts with water to form 2-aminoadipate 6-semialdehyde ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.7 37
-
 assay at Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.7 8.5
-
 assay at Penicillium chrysogenum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.7 NAD+
-
 Penicillium chrysogenum
1.5.1.10 NADPH
-
 Penicillium chrysogenum