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Literature summary extracted from

  • Brandsch, R.; Bichler, V.; Mauch, L.; Decker, K.
    Cysteine to serine replacements in 6-hydroxy-D-nicotine oxidase. Consequences for enzyme activity, cofactor incorporation, and formation of high molecular weight protein complexes with molecular chaperones (GroEL) (1993), J. Biol. Chem., 268, 12724-12729.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.6 expressed in Escherichia coli JM109 Paenarthrobacter nicotinovorans

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.6 C136S 5% of wild-type activity, slight activation with glycerol-3-phosphate and FAD Paenarthrobacter nicotinovorans
1.5.3.6 C136S/C260S no activity, no activation with glycerol-3-phosphate and FAD Paenarthrobacter nicotinovorans
1.5.3.6 C260S 15% of wild-type activity, no activation with glycerol-3-phosphate and FAD Paenarthrobacter nicotinovorans
1.5.3.6 C433S 40% of wild-type activity, 50% activation with glycerol-3-phosphate and FAD Paenarthrobacter nicotinovorans
1.5.3.6 C433S deletions F448 and R449, no activity, no activation with glycerol-3-phosphate and FAD Paenarthrobacter nicotinovorans
1.5.3.6 C59S 40% of wild-type activity, no activation with glycerol-3-phosphate and FAD Paenarthrobacter nicotinovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.6 Dithionitrobenzoic acid inhibits holoenzyme formation from apoenzyme and FAD, can be prevented by addition of 2-mercaptoethanol Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.6 Paenarthrobacter nicotinovorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.6 FAD flavinylation can be performed with purified apoenzyme post-translationally with chaperonins ATP, GroEL and GroES Paenarthrobacter nicotinovorans