Literature summary extracted from
Stoltz, M.; Rysavy, P.; Kalousek, F.; Brandsch, R.
Folding, flavinylation, and mitochondrial import of 6-hydroxy-D-nicotine oxidase fused to the presequence of rat dimethylglycine dehydrogenase (1995), J. Biol. Chem., 270, 8016-8022.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.5.3.6 |
glycerol-3-phosphate |
activation of autocatalytical flavinylation of the enzyme in rabbit reticulocyte lysate |
Paenarthrobacter nicotinovorans |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.5.3.6 |
expressed in Escherichia coli JM109 as a fusion protein with dimethylglycine dehydrogenase and translated into rabbit reticulocyte lysate |
Paenarthrobacter nicotinovorans |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.5.3.6 |
(R)-6-hydroxynicotine + H2O + O2 |
Paenarthrobacter nicotinovorans |
- |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.5.3.6 |
Paenarthrobacter nicotinovorans |
- |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.5.3.6 |
(R)-6-hydroxynicotine + H2O + O2 |
- |
Paenarthrobacter nicotinovorans |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 |
- |
? |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.5.3.6 |
FAD |
formation of the bond to FAD proceeds autocatalytically |
Paenarthrobacter nicotinovorans |
|
1.5.3.6 |
FAD |
covalently bound to a histidine |
Paenarthrobacter nicotinovorans |
|