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Literature summary extracted from

  • Decker, K.; Bleeg, H.
    Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans (1965), Biochim. Biophys. Acta, 105, 313-324.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.5 6-hydroxy-D-nicotine 50% inhibition at 0.18 mM Paenarthrobacter nicotinovorans
1.5.3.6 6-hydroxy-L-nicotine at 0.12 M 50% inhibition Paenarthrobacter nicotinovorans
1.5.99.4 additional information not inhibited by any intermediates Pseudarthrobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.5 0.02
-
 6-hydroxy-L-nicotine
-
 Paenarthrobacter nicotinovorans
1.5.3.6 0.1
-
 6-hydroxy-D-nicotine
-
 Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.5 (S)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.99.4 nicotine + acceptor + H2O Pseudarthrobacter oxydans first enzyme in the nicotine degradation pathway 6-hydroxynicotine + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.5 Paenarthrobacter nicotinovorans
-
 formerly Arthrobacter oxidans
-
1.5.3.6 Paenarthrobacter nicotinovorans
-
-
-
1.5.99.4 Pseudarthrobacter oxydans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.5 partially Paenarthrobacter nicotinovorans
1.5.99.4 protamine sulfate and ammonium sulfate precipitation, gel filtration, ion-exchange Pseudarthrobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.4 additional information
-
 0.119 units per mg protein, unit is defined as the amount causing a decrease in absorbancy at 578 nm of 0.001 per min Pseudarthrobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.5 (S)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.5 L-6-hydroxy-nor-nicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-aminobutan-1-one + H2O2
-
 ?
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.6 6-hydroxy-nornicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 4-amino-1-(6-hydroxypyridin-3-yl)-butan-1-one + H2O2
-
 ?
1.5.99.4 nicotine + acceptor + H2O non-stereospecific enzyme if grown on L-nicotine, assayed with 2,6-dichlorophenolindophenol Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
 ?
1.5.99.4 nicotine + acceptor + H2O first enzyme in the nicotine degradation pathway Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.99.4 25
-
 assay at Pseudarthrobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.5 7.5
-
 assay at Paenarthrobacter nicotinovorans
1.5.99.4 6.8
-
 assay at Pseudarthrobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.5 FAD
-
 Paenarthrobacter nicotinovorans
1.5.3.6 FAD
-
 Paenarthrobacter nicotinovorans