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Literature summary extracted from

  • Decker, K.; Dai, V.D.; Möhler, H.; Bruhmuller, M.
    D- and L-6-hydroxynicotine oxidase, enantioenzymes of Arthrobacter oxidans (1972), Z. Naturforsch. B, 27, 1072-1073.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.3.6 2,6-dichlorophenolindophenol the rate of the overall process is higher than with oxygen Paenarthrobacter nicotinovorans
1.5.3.6 methylene blue the rate of the overall process is higher than with oxygen Paenarthrobacter nicotinovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.5 6-hydroxy-D-nicotine competitive inhibitor Paenarthrobacter nicotinovorans
1.5.3.5 methylene blue strong inhibitor Paenarthrobacter nicotinovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.5 0.000082
-
 FAD
-
 Paenarthrobacter nicotinovorans
1.5.3.5 0.02
-
 6-hydroxy-L-nicotine
-
 Paenarthrobacter nicotinovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.5 93000
-
 sedimentation equilibrium Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.5 (S)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.6 additional information Paenarthrobacter nicotinovorans enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.5 Paenarthrobacter nicotinovorans
-
 formerly Arthrobacter oxidans
-
1.5.3.6 Paenarthrobacter nicotinovorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.5 (S)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 transcient product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously ?
1.5.3.6 additional information enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen Paenarthrobacter nicotinovorans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.6 monomer
-
 Paenarthrobacter nicotinovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.5 29.3
-
 6-hydroxy-L-nicotine
-
 Paenarthrobacter nicotinovorans
1.5.3.6 19.8
-
 6-hydroxy-D-nicotine 30°C pH 9.2 Paenarthrobacter nicotinovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.5 FAD 1 FAD per subunit, non-covalently bound Paenarthrobacter nicotinovorans
1.5.3.6 FAD one mol FAD per mol enzyme Paenarthrobacter nicotinovorans
1.5.3.6 FAD covalently bound to a histidine Paenarthrobacter nicotinovorans