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Literature summary extracted from

  • Hinkkanen, A.; Lilius, E.M.; Nowack, J.; Maas, R.; Decker, K.
    Purification of the flavoproteins 6-hydroxy-D- and 6-hydroxy-L-nicotine oxidase using hydrophobic affinity chromatography (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 801-806.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.6 50000
-
 SDS-PAGE Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.5 (S)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.5 Paenarthrobacter nicotinovorans
-
 formerly Arthrobacter oxidans
-
1.5.3.6 Paenarthrobacter nicotinovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.5
-
 Paenarthrobacter nicotinovorans
1.5.3.6
-
 Paenarthrobacter nicotinovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.6 28
-
-
 Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.5 (S)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.5 homodimer
-
 Paenarthrobacter nicotinovorans
1.5.3.6 monomer
-
 Paenarthrobacter nicotinovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.5 FAD 1 FAD per subunit, non-covalently bound Paenarthrobacter nicotinovorans
1.5.3.6 FAD
-
 Paenarthrobacter nicotinovorans