Any feedback?
Literature summary extracted from
- Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans (1994), Mol. Microbiol., 13, 929-936.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.5 | MoO42- | induces enzyme expression activity | Paenarthrobacter nicotinovorans |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.99.4 | - |
Paenarthrobacter nicotinovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.4 | Tungsten | inhibitory to enzyme activity, does not inhibit enzyme synthesis | Paenarthrobacter nicotinovorans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.5.99.4 | membrane | present both as soluble and membrane-associated form | Paenarthrobacter nicotinovorans | 16020 | - |
| 1.5.99.4 | soluble | present both as soluble and membrane-associated form | Paenarthrobacter nicotinovorans | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.6 | additional information | molybdate is not required for the induction of 6-hydroxy-D-nicotine | Paenarthrobacter nicotinovorans | |
| 1.5.99.4 | Iron | subunit B carries an iron-sulfur cluster | Paenarthrobacter nicotinovorans | |
| 1.5.99.4 | Molybdenum | subunit C contains a molybdopterin-binding domain | Paenarthrobacter nicotinovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.4 | 14924 | - |
x * 30011, subunit A, x * 14924, subunit B, x * 87677, subunit C, calculated | Paenarthrobacter nicotinovorans |
| 1.5.99.4 | 30011 | - |
x * 30011, subunit A, x * 14924, subunit B, x * 87677, subunit C, calculated | Paenarthrobacter nicotinovorans |
| 1.5.99.4 | 87677 | - |
x * 30011, subunit A, x * 14924, subunit B, x * 87677, subunit C, calculated | Paenarthrobacter nicotinovorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.5 | (S)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously | ? | |
| 1.5.3.5 | additional information | Paenarthrobacter nicotinovorans | enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen | ? | - |
? | |
| 1.5.3.6 | (R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.3.5 | Paenarthrobacter nicotinovorans | - |
formerly Arthrobacter oxidans | - |
| 1.5.3.6 | Paenarthrobacter nicotinovorans | - |
- |
- |
| 1.5.99.4 | Paenarthrobacter nicotinovorans | Q93NH4 | encoded on megaplasmid pAO1 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.5 | (S)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously | ? | |
| 1.5.3.5 | additional information | enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen | Paenarthrobacter nicotinovorans | ? | - |
? | |
| 1.5.3.6 | (R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.3.5 | homodimer | - |
Paenarthrobacter nicotinovorans |
| 1.5.3.6 | monomer | - |
Paenarthrobacter nicotinovorans |
| 1.5.99.4 | ? | x * 30011, subunit A, x * 14924, subunit B, x * 87677, subunit C, calculated | Paenarthrobacter nicotinovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.99.4 | NDH | - |
Paenarthrobacter nicotinovorans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.5 | FAD | 1 FAD per subunit, non-covalently bound | Paenarthrobacter nicotinovorans | |
| 1.5.3.6 | FAD | FAD is bound via an (8alpha)-isoalloxazine-(N3)histidyl linkage | Paenarthrobacter nicotinovorans | |
| 1.5.3.6 | FAD | covalently bound to a histidine | Paenarthrobacter nicotinovorans | |
| 1.5.99.4 | FAD | subunit A binds FAD | Paenarthrobacter nicotinovorans | |
| 1.5.99.4 | iron-sulfur centre | subunit B carries an iron-sulfur cluster | Paenarthrobacter nicotinovorans | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.5.99.4 | Paenarthrobacter nicotinovorans | expression of all subunits requires the presence of nicotine and molybdenum in the culture medium | up |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
