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Literature summary extracted from

  • Paik, W.K.; Kim, S.
    epsilon-Alkyllysinase. New assay method, purification, and biological significance (1974), Arch. Biochem. Biophys., 165, 369-378.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.3.4 10% glycerol protects enzyme from inactivation during Sephadex G-200 column chromatography Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.4 2,6-dichlorophenolindophenol
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.4 1.05
-
 N6-methyl-L-lysine crude extract Rattus norvegicus
1.5.3.4 1.9
-
 N6-methyl-L-lysine partially purified enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.3.4 mitochondrion exclusive localization Rattus norvegicus 5739
-
1.5.3.4 particle-bound
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.4 100000 200000 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.4 16fold partial purification Rattus norvegicus
1.5.3.4 enzyme from kidney Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.3.4 N6-methyl-L-lysine + H2O + O2 = L-lysine + formaldehyde + H2O2 mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.4 kidney low enzyme activity in young rat, increases to the adult level, highest level of demethylation activity Rattus norvegicus
-
1.5.3.4 liver low enzyme activity in young rat, increases to the adult level, after kidney next highest level of demethylation activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.4 additional information
-
 values in dependence of different incubation mixtures Rattus norvegicus
1.5.3.4 0.0283
-
 11fold purified enzyme Rattus norvegicus
1.5.3.4 0.0433
-
 16fold purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.4 6-(methylamino)-2-oxohexanoic acid + H2O + O2 free alpha-amino group is not required for susceptibility Rattus norvegicus 6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
 ?
1.5.3.4 additional information not: delta-N-monomethyl-L-ornithine Rattus norvegicus ?
-
 ?
1.5.3.4 additional information demethylation involves an electron transport system: FAD is the physiological electron acceptor, phenazine methosulfate as artificial and more efficient electron acceptor than FAD, final electron acceptor is oxygen or 2,6-dichlorophenolindophenol Rattus norvegicus ?
-
 ?
1.5.3.4 N6,N6-dimethyl-L-lysine + O2 + H2O
-
 Rattus norvegicus L-lysine + formaldehyde + H2O2
-
 ?
1.5.3.4 N6-methyl-D-lysine + H2O + O2 11% of the activity with epsilon-N-methyl-L-lysine Rattus norvegicus D-lysine + formaldehyde + H2O2
-
 ?
1.5.3.4 N6-methyl-L-lysine + O2 + H2O reaction in reverse direction, i.e. methylation of L-lysine, is probably catalyzed by protein methylase III Rattus norvegicus L-lysine + formaldehyde + H2O2 equimolar amounts of L-lysine and formaldehyde ir
1.5.3.4 N6-methyl-L-lysine + O2 + H2O enzyme consumes per mol N6-methyl-L-lysine half a mol of oxygen Rattus norvegicus L-lysine + formaldehyde + H2O2 equimolar amounts of L-lysine and formaldehyde ir

Synonyms

EC Number Synonyms Comment Organism
1.5.3.4 More identical to histone demethylase Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.4 55
-
 4 min: 70% loss of activity, 10 min: complete loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.4 7.2
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.4 6 8 pH 6: about 35% of activity maximum, pH 8: about 50% of activity maximum Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.4 FAD physiological electron acceptor, not required with whole homogenate, but required for partially purified enzyme Rattus norvegicus