EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.8 | cloning of cDNA encoding the bifunctional protein with lysine 2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, expression of the single gene is subject to spatial and developmental controls, expression in Escherichia coli produces no active LKR, perhaps because of a missing post-translational modification in prokaryotes, expression in Saccharomyces cerevisiae mutant lys1 | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | 117000 | - |
x * 117000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, calculated from cDNA sequence | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Saccharomyces cerevisiae | plays essential role in lysine biosynthesis, LKR and SDH are separate polypeptides | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Arabidopsis thaliana | first enzyme in lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Arabidopsis thaliana | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.8 | Arabidopsis thaliana | - |
var. C24 | - |
1.5.1.8 | Arabidopsis thaliana | - |
ecotype Landsberg erecta and Columbia | - |
1.5.1.8 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.5.1.8 | additional information | enzyme may be activated by post-translational modification | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.8 | embryo | - |
Arabidopsis thaliana | - |
1.5.1.8 | endosperm | outer layers | Arabidopsis thaliana | - |
1.5.1.8 | flower | LKR mRNA is highly abundant in ovules and vascular tissue of anther filaments | Arabidopsis thaliana | - |
1.5.1.8 | additional information | not in pollen grains | Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | - |
Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Arabidopsis thaliana | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | plays essential role in lysine biosynthesis, LKR and SDH are separate polypeptides | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | first enzyme in lysine catabolism | Arabidopsis thaliana | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Arabidopsis thaliana | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Arabidopsis thaliana | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.8 | ? | x * 117000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, calculated from cDNA sequence | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.8 | NADPH | - |
Arabidopsis thaliana |