EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.8 | additional information | high-Lys opaque-2 mutant has significantly reduced LKR activity | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.8 | Alkaline phosphatase | reduces LKR activity | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.8 | 3.7 | - |
2-oxoglutarate | - |
Glycine max | |
1.5.1.8 | 13.7 | - |
L-lysine | - |
Glycine max |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | 123000 | - |
2 * 123000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, additional 100 kDa band is a proteolytic cleavage product, SDS-PAGE | Glycine max |
1.5.1.8 | 256000 | - |
gel filtration, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Zea mays | - |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Oryza sativa | - |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Glycine max | first and possibly a rate-limiting step in lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Glycine max | enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.8 | Glycine max | - |
- |
- |
1.5.1.8 | Oryza sativa | - |
- |
- |
1.5.1.8 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.8 | 1630.5fold purification of a bifunctional protein containing lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.8 | seed | developing | Zea mays | - |
1.5.1.8 | seed | developing | Glycine max | - |
1.5.1.8 | seed | developing | Oryza sativa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | 3.261 | - |
- |
Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | - |
Zea mays | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | - |
Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Zea mays | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Glycine max | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | first and possibly a rate-limiting step in lysine catabolism | Glycine max | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation | Glycine max | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Zea mays | ? | - |
? | |
1.5.1.8 | additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Glycine max | ? | - |
? | |
1.5.1.8 | additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.8 | homodimer | - |
Zea mays |
1.5.1.8 | homodimer | - |
Oryza sativa |
1.5.1.8 | homodimer | 2 * 123000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, additional 100 kDa band is a proteolytic cleavage product, SDS-PAGE | Glycine max |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | 30 | - |
assay at | Zea mays |
1.5.1.8 | 30 | - |
assay at | Glycine max |
1.5.1.8 | 30 | - |
assay at | Oryza sativa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | 7.2 | - |
- |
Glycine max |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.8 | NADPH | - |
Zea mays | |
1.5.1.8 | NADPH | - |
Glycine max | |
1.5.1.8 | NADPH | - |
Oryza sativa |