EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.8 | abscisic acid | 0.05 mM, incubation for 12 h, significantly increased activity | Brassica napus | |
1.5.1.8 | additional information | activity is enhanced with decreasing osmotic potential and LKR/SDH gene is upregulated by osmotic stress | Brassica napus | |
1.5.1.8 | polyethylene glycol 6000 | enzyme activity increases in response to an upshock osmotic stress caused by polyethylene glycol 6000 solutions of decreasing osmotic potential with values ranging from -0.1 to -3 MPa | Brassica napus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.8 | isolation of the cDNA and gene encoding bifunctional enzyme with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Brassica napus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Brassica napus | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Brassica napus | L-lysine catabolism is reversibly osmo-regulated at the level of LKR/SDH bifunctional enzyme | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.8 | Brassica napus | - |
var. oleifera cv. Samourai | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.8 | leaf | - |
Brassica napus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Brassica napus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Brassica napus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | L-lysine catabolism is reversibly osmo-regulated at the level of LKR/SDH bifunctional enzyme | Brassica napus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | 30 | - |
assay at | Brassica napus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.8 | additional information | NADH can not replace NADPH | Brassica napus | |
1.5.1.8 | NADPH | - |
Brassica napus |