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Literature summary extracted from

  • Gaziola, S.A.; Teixeira, C.M.; Lugli, J.; Sodek, L.; Azevedo, R.A.
    The enzymology of lysine catabolism in rice seeds. Isolation, characterization, and regulatory properties of a lysine 2-oxoglutarate reductase/saccharopine dehydrogenase bifunctional polypeptide (1997), Eur. J. Biochem., 247, 364-371.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.8 presence of 5% w/v insoluble polyvinylpyrrolidone and 10% v/v glycerol in extraction buffer and glycerol in running buffers is crucial to maintain enzyme activity Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.8 saccharopine product inhibition, competitive with 2-oxoglutarate, noncompetitive with L-lysine Oryza sativa
1.5.1.9 2-Aminoadipate strong inhibition at 5 mM Oryza sativa
1.5.1.9 2-oxoglutarate
-
Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.8 0.032
-
NADPH
-
Oryza sativa
1.5.1.8 0.79
-
2-oxoglutarate
-
Oryza sativa
1.5.1.8 4.48
-
L-lysine
-
Oryza sativa
1.5.1.9 0.13
-
saccharopine
-
Oryza sativa
1.5.1.9 0.49
-
NAD+
-
Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.8 202000
-
non-denaturing PAGE, bifunctional protein containing lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, one band represents the holoenzyme and shows higher enzyme activities compared to a second band of 396 kDa with much lower enzyme activities indicating a multimeric structure Oryza sativa
1.5.1.8 203000
-
gel filtration, bifunctional protein containing lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa
1.5.1.9 202000
-
native non-denaturing gel, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Oryza sativa
1.5.1.9 203000
-
gel filtration with Sephacryl S-200, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Oryza sativa
1.5.1.9 396000
-
native non-denaturing gel, low activity compared to 202000 kDa band Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Oryza sativa regulation of lysine catabolism may be essential for lysine accumulation in rice plants N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Oryza sativa lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Oryza sativa IAC 165 regulation of lysine catabolism may be essential for lysine accumulation in rice plants N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Oryza sativa IAC 165 lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Oryza sativa involved in lysine catabolism L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.8 Oryza sativa
-
IAC 165
-
1.5.1.8 Oryza sativa IAC 165
-
IAC 165
-
1.5.1.9 Oryza sativa
-
rice var IAC-165
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.8 38fold purification Oryza sativa
1.5.1.8 partial purification of a single bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa
1.5.1.9 lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Oryza sativa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.8 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH + H+ ordered sequence mechanism, where 2-oxoglutarate is first substrate and saccharopine is last product Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.8 seed developing Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.8 additional information
-
-
Oryza sativa
1.5.1.9 0.0017
-
crude extract from immature seeds Oryza sativa
1.5.1.9 0.052
-
after purification Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH regulation of lysine catabolism may be essential for lysine accumulation in rice plants Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH lysine catabolism Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH regulation of lysine catabolism may be essential for lysine accumulation in rice plants Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH lysine catabolism Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.8 additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa ?
-
?
1.5.1.8 additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa IAC 165 ?
-
?
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
Oryza sativa L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine catabolism Oryza sativa L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
Oryza sativa IAC 165 L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.5.1.8 More 369 kDa band may represent a dimeric or tetrameric molecule, multimeric form results from the association of the 202 kDa LOR/SDH band Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.5.1.9 SDH
-
Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.8 30
-
assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.8 NADPH
-
Oryza sativa
1.5.1.9 NAD+
-
Oryza sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.8 2
-
saccharopine
-
Oryza sativa