EC Number | General Stability | Organism |
---|---|---|
1.5.1.8 | presence of 5% w/v insoluble polyvinylpyrrolidone and 10% v/v glycerol in extraction buffer and glycerol in running buffers is crucial to maintain enzyme activity | Oryza sativa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.8 | saccharopine | product inhibition, competitive with 2-oxoglutarate, noncompetitive with L-lysine | Oryza sativa | |
1.5.1.9 | 2-Aminoadipate | strong inhibition at 5 mM | Oryza sativa | |
1.5.1.9 | 2-oxoglutarate | - |
Oryza sativa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.8 | 0.032 | - |
NADPH | - |
Oryza sativa | |
1.5.1.8 | 0.79 | - |
2-oxoglutarate | - |
Oryza sativa | |
1.5.1.8 | 4.48 | - |
L-lysine | - |
Oryza sativa | |
1.5.1.9 | 0.13 | - |
saccharopine | - |
Oryza sativa | |
1.5.1.9 | 0.49 | - |
NAD+ | - |
Oryza sativa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | 202000 | - |
non-denaturing PAGE, bifunctional protein containing lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, one band represents the holoenzyme and shows higher enzyme activities compared to a second band of 396 kDa with much lower enzyme activities indicating a multimeric structure | Oryza sativa |
1.5.1.8 | 203000 | - |
gel filtration, bifunctional protein containing lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa |
1.5.1.9 | 202000 | - |
native non-denaturing gel, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein | Oryza sativa |
1.5.1.9 | 203000 | - |
gel filtration with Sephacryl S-200, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein | Oryza sativa |
1.5.1.9 | 396000 | - |
native non-denaturing gel, low activity compared to 202000 kDa band | Oryza sativa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Oryza sativa | regulation of lysine catabolism may be essential for lysine accumulation in rice plants | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Oryza sativa | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Oryza sativa IAC 165 | regulation of lysine catabolism may be essential for lysine accumulation in rice plants | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | Oryza sativa IAC 165 | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.9 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | Oryza sativa | involved in lysine catabolism | L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.8 | Oryza sativa | - |
IAC 165 | - |
1.5.1.8 | Oryza sativa IAC 165 | - |
IAC 165 | - |
1.5.1.9 | Oryza sativa | - |
rice var IAC-165 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.8 | 38fold purification | Oryza sativa |
1.5.1.8 | partial purification of a single bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa |
1.5.1.9 | lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein | Oryza sativa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.5.1.8 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH + H+ | ordered sequence mechanism, where 2-oxoglutarate is first substrate and saccharopine is last product | Oryza sativa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.8 | seed | developing | Oryza sativa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | additional information | - |
- |
Oryza sativa |
1.5.1.9 | 0.0017 | - |
crude extract from immature seeds | Oryza sativa |
1.5.1.9 | 0.052 | - |
after purification | Oryza sativa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | regulation of lysine catabolism may be essential for lysine accumulation in rice plants | Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Oryza sativa IAC 165 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | regulation of lysine catabolism may be essential for lysine accumulation in rice plants | Oryza sativa IAC 165 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Oryza sativa IAC 165 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.8 | additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa | ? | - |
? | |
1.5.1.8 | additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa IAC 165 | ? | - |
? | |
1.5.1.9 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Oryza sativa | L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+ | - |
r | |
1.5.1.9 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | involved in lysine catabolism | Oryza sativa | L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+ | - |
r | |
1.5.1.9 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Oryza sativa IAC 165 | L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.8 | More | 369 kDa band may represent a dimeric or tetrameric molecule, multimeric form results from the association of the 202 kDa LOR/SDH band | Oryza sativa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.9 | SDH | - |
Oryza sativa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.8 | 30 | - |
assay at | Oryza sativa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.8 | NADPH | - |
Oryza sativa | |
1.5.1.9 | NAD+ | - |
Oryza sativa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.8 | 2 | - |
saccharopine | - |
Oryza sativa |