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Literature summary extracted from

  • Gaziola, S.A.; Sodek, L.; Arruda, P.; Lea, P.J.; Azevedo, R.A.
    Degradation of lysine in rice seeds: Effect of calcium, ionic strength, S-adenosylmethionine and S-2-aminoethyl-L-cysteine on the lysine 2-oxoglutarate reductase-saccharopine dehydrogenase bifunctional enzyme (2000), Physiol. Plant., 110, 164-171.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.8 organic solvent activates Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.8 genes encoding bifunctional protein with lysine 2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities are cloned Zea mays
1.5.1.8 genes encoding bifunctional protein with lysine 2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities are cloned Arabidopsis thaliana

General Stability

EC Number General Stability Organism
1.5.1.8 stabilization of holoenzyme by high salt concentrations, e.g. KCl Zea mays
1.5.1.8 stabilization of holoenzyme by high salt concentrations, e.g. KCl Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.8 EGTA 1 mM, 21% loss of activity, addition of 1.6 mM CaCl2 increases activity by 43% Oryza sativa
1.5.1.8 EGTA up to 70% inhibition, dependent on salt concentration of buffer Zea mays
1.5.1.8 S-2-aminoethyl-L-cysteine inhibits when present in assay with L-lysine, possibly competitive with L-lysine Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.8 17
-
 S-2-aminoethyl-L-cysteine
-
 Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.8 Ca2+ activates, lysine 2-oxoglutarate reductase activity is modulated by Ca2+, possible modulation of an inhibitory domain Zea mays
1.5.1.8 Ca2+ activates, lysine 2-oxoglutarate reductase activity is modulated by Ca2+, possible modulation of an inhibitory domain Oryza sativa
1.5.1.8 Ca2+ 1.6 mM, activates Oryza sativa
1.5.1.8 Mg2+ activates Zea mays
1.5.1.8 Salt activated by high salt concentrations Zea mays
1.5.1.8 Salt activated by high salt concentrations Oryza sativa
1.5.1.8 Salt lysine 2-oxoglutarate reductase domain is modulated by ionic strength Zea mays
1.5.1.8 Salt lysine 2-oxoglutarate reductase domain is modulated by ionic strength Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Zea mays lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Arabidopsis thaliana lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Oryza sativa lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Oryza sativa IAC 165 lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.8 Arabidopsis thaliana
-
-
-
1.5.1.8 Oryza sativa
-
 IAC 165
-
1.5.1.8 Oryza sativa IAC 165
-
 IAC 165
-
1.5.1.8 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.8 partial purification of a single bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.8 endosperm
-
 Arabidopsis thaliana
-
1.5.1.8 endosperm located specifically in Zea mays
-
1.5.1.8 endosperm located specifically in Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.8 additional information
-
-
 Zea mays
1.5.1.8 additional information
-
-
 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Zea mays N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH lysine catabolism Zea mays N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH lysine catabolism Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH lysine catabolism Oryza sativa N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH lysine catabolism Oryza sativa IAC 165 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.8 additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Zea mays ?
-
 ?
1.5.1.8 additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Arabidopsis thaliana ?
-
 ?
1.5.1.8 additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa ?
-
 ?
1.5.1.8 additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Oryza sativa IAC 165 ?
-
 ?
1.5.1.8 S-2-aminoethyl-L-cysteine + 2-oxoglutarate + NADPH 52% of activity with equimolar concentration of L-lysine, same Vmax, but higher Km-value than for L-lysine Oryza sativa ?
-
 ?
1.5.1.8 S-2-aminoethyl-L-cysteine + 2-oxoglutarate + NADPH 52% of activity with equimolar concentration of L-lysine, same Vmax, but higher Km-value than for L-lysine Oryza sativa IAC 165 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.8 additional information
-
 additional information lower turnover number with S-2-aminoethyl-L-cysteine as substrate than with L-lysine Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.8 7
-
-
 Zea mays
1.5.1.8 7
-
-
 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.8 NADPH
-
 Zea mays
1.5.1.8 NADPH
-
 Arabidopsis thaliana
1.5.1.8 NADPH
-
 Oryza sativa