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Literature summary extracted from

  • Hutzler, J.; Dancis, J.
    Conversion of lysine to saccharopine by human tissues (1968), Biochim. Biophys. Acta, 158, 62-69.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.8 medicine hyperlysinemia Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.8 (NH4)2SO4 1 mM, 71% inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Homo sapiens lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.8 9.6fold partial purification from liver Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.8 adrenal gland low enzyme concentration Homo sapiens
-
1.5.1.8 brain low enzyme concentration Homo sapiens
-
1.5.1.8 heart high enzyme concentration Homo sapiens
-
1.5.1.8 kidney after liver next highest enzyme concentration Homo sapiens
-
1.5.1.8 liver highest enzyme concentration Homo sapiens
-
1.5.1.8 additional information trace enzyme activity in lung, spleen, and striated muscle, no activity in pancreas, thymus, peripheral leucocyte or erythrocyte Homo sapiens
-
1.5.1.8 skin low enzyme concentration Homo sapiens
-
1.5.1.8 thyroid gland low enzyme concentration Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.8 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.5.1.8 -25°C, 1 month, stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O epsilon-N-(L-glutaryl-2)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O epsilon-N-(L-glutaryl-2)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH lysine catabolism Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.8 6.7 7
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.8 6.2 8.6 pH 6.2: about 50% of activity maximum, pH 8.6: about 60% of activity maximum Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.8 additional information NADH can not replace NADPH Homo sapiens
1.5.1.8 NADPH specific for NADPH Homo sapiens