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Literature summary extracted from

  • Davis, A.T.
    Assay for lysine-ketoglutarate reductase by reversed-phase high-performance liquid chromatography (1989), J. Chromatogr., 497, 263-267.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Rattus norvegicus saccharopine pathway is the major route of lysine breakdown N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Rattus norvegicus lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.8 Rattus norvegicus
-
 pregnant Sprague-Dawley rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.8 additional information
-
 HPLC lysine-ketoglutarate reductase assay Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH saccharopine pathway is the major route of lysine breakdown Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH lysine catabolism Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.8 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.8 NADP+
-
 Rattus norvegicus
1.5.1.8 NADPH
-
 Rattus norvegicus