Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Scrutton, M.C.; Beis, I.
    Inhibitory effects of histidine and their reversal. The roles of pyruvate carboxylase and N10-formyltetrahydrofolate dehydrogenase (1979), Biochem. J., 177, 833-846.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.6 additional information not activated by methionine or ethionine Rattus norvegicus
1.5.1.6 NADPH activates at concentrations and in ratio with NADP+ consistent with those in rat liver in vivo, activation is associated with higher Ki-value for tetrahydrofolate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.6 additional information not inhibited by NADPH Rattus norvegicus
1.5.1.6 tetrahydrofolate competitive to 10-formyltetrahydrofolate and non-competitive to NADP+; very strong product inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.6 0.00092
-
 NADP+ at pH 7 Rattus norvegicus
1.5.1.6 0.0045
-
 10-formyltetrahydrofolate at pH 7 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.6 108000
-
 4 * 108000, SDS-PAGE Rattus norvegicus
1.5.1.6 413000
-
 gel filtration, sedimentation analysis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6 250fold purification Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ sequential mechanism of substrate addition Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.6 kidney less activity Rattus norvegicus
-
1.5.1.6 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.6 0.7 0.8 at 25°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.5.1.6 -70°C, 0.05 M phosphate buffer, pH 7.2, 50%-saturated (NH4)2SO4, 20% glycerol, 1 mM EDTA, 0.1 mM dithioerythritol, 0.01 mM phenylmethanesulfonyl fluoride, several months, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 additional information hydrolysis catalyzed by enzyme at 21% of the rate of CO2 formation Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.6 homotetramer 4 * 108000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.6 additional information
-
 enzyme activity at 37°C is twice that at 25°C Rattus norvegicus
1.5.1.6 37
-
 maximal catalytic capacity at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
1.5.1.6 NADP+ specific for Rattus norvegicus
1.5.1.6 NADP+ NADP+-dependent Rattus norvegicus
1.5.1.6 tetrahydrofolate
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.6 0.007
-
 tetrahydrofolate (-)-isomer Rattus norvegicus