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Literature summary extracted from

  • Johlin, F.C.; Swain, E.; Smith, C.; Tephly, T.R.
    Studies on the mechanism of methanol poisoning: purification and comparison of rat and human liver 10-formyltetrahydrofolate dehydrogenase (1989), Mol. Pharmacol., 35, 745-750.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.6 medicine formic acidemia: toxicity of methanol in humans is correlated with formate accumulation as a result of low rates of formate oxidation, which is in vivo dependent on enzyme activity and on hepatic tetrahydrofolate levels Homo sapiens

General Stability

EC Number General Stability Organism
1.5.1.6 in general activity is labile Homo sapiens
1.5.1.6 in general activity is labile Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.6 0.02
-
 10-formyltetrahydrofolate
-
 Rattus norvegicus
1.5.1.6 0.05
-
 10-formyltetrahydrofolate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.6 cytosol
-
 Homo sapiens 5829
-
1.5.1.6 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.6 96000
-
 x * 96000, SDS-PAGE Homo sapiens
1.5.1.6 96000
-
 x * 96000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O Rattus norvegicus
-
 tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O Homo sapiens folate-dependent pathway is the exclusive route of formate metabolism in primates, plays major role in disposition of formate generated from one-carbon moieties of environmental chemicals, drugs, and foods, importance in the toxicity of methanol tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Homo sapiens
-
-
-
1.5.1.6 Rattus norvegicus
-
 Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6 free of 10-formyltetrahydrofolate hydrolase activity Homo sapiens
1.5.1.6 free of 10-formyltetrahydrofolate hydrolase activity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.6 liver activity in human liver is 26% of that in rat liver, reduced amount of enzyme in human liver Homo sapiens
-
1.5.1.6 liver activity in human liver is 26% of that in rat liver, reduced amount of enzyme in human liver Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.6 additional information
-
-
 Homo sapiens
1.5.1.6 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O
-
 Homo sapiens tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O
-
 Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O folate-dependent pathway is the exclusive route of formate metabolism in primates, plays major role in disposition of formate generated from one-carbon moieties of environmental chemicals, drugs, and foods, importance in the toxicity of methanol Homo sapiens tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.6 ? x * 96000, SDS-PAGE Homo sapiens
1.5.1.6 ? x * 96000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.6 additional information
-
 assay at room temperature Homo sapiens
1.5.1.6 additional information
-
 assay at room temperature Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Homo sapiens
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
1.5.1.6 NADP+
-
 Homo sapiens
1.5.1.6 NADP+
-
 Rattus norvegicus
1.5.1.6 tetrahydrofolate
-
 Homo sapiens
1.5.1.6 tetrahydrofolate
-
 Rattus norvegicus