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Literature summary extracted from

  • Min, H.; Shane, B.; Stokstad, E.L.R.
    Identification of 10-formyltetrahydrofolate dehydrogenase-hydrolase as a major folate binding protein in liver cytosol (1988), Biochim. Biophys. Acta, 967, 348-353.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.6 tetrahydrofolate
-
 Rattus norvegicus
1.5.1.6 tetrahydrofolate
-
 Sus scrofa
1.5.1.6 tetrahydropteroylhexaglutamate 1 mol per mol of tetrameric protein, potent product inhibitor: 0.0003 mM, 90% inhibition Sus scrofa
1.5.1.6 tetrahydropteroylpolyglutamate potent product inhibitor Rattus norvegicus
1.5.1.6 tetrahydropteroylpolyglutamate inhibition increases with increasing length of polyglutamyl chain; potent product inhibitor Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.6 additional information
-
 additional information
-
 Sus scrofa
1.5.1.6 0.007
-
 10-formyltetrahydrofolate
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.6 91000
-
 4 * 91000, three minor bands of 63000, 58000 and 39000, SDS-PAGE Sus scrofa
1.5.1.6 390000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O Rattus norvegicus disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O Sus scrofa disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Rattus norvegicus
-
 male sprague-dawley rats
-
1.5.1.6 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6
-
 Rattus norvegicus
1.5.1.6
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.6 liver
-
 Rattus norvegicus
-
1.5.1.6 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.6 0.51
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O
-
 Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+ very tight binding of product, binds its product rather than its substrate ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O
-
 Sus scrofa tetrahydrofolate + CO2 + NADPH + H+ very tight binding of product, binds its product rather than its substrate ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydropteroylhexaglutamate + NADP+ + H2O
-
 Sus scrofa tetrahydropteroylhexaglutamate + CO2 + NADPH
-
 ?
1.5.1.6 10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O
-
 Rattus norvegicus tetrahydropteroylpolyglutamate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O affinity increases with increasing length of polyglutamyl chain Sus scrofa tetrahydropteroylpolyglutamate + CO2 + NADPH + H+
-
 ?
1.5.1.6 additional information bifunctional protein: 10-formyltetrahydrofolate dehydrogenase/hydrolase, hydrolysis at 20-30% of the oxidative rate Rattus norvegicus ?
-
 ?
1.5.1.6 additional information bifunctional protein: 10-formyltetrahydrofolate dehydrogenase/hydrolase, hydrolysis at 20-30% of the oxidative rate Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.6 homotetramer
-
 Rattus norvegicus
1.5.1.6 homotetramer 4 * 91000, three minor bands of 63000, 58000 and 39000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.5.1.6 More identical with folate binding protein cytosol-I, FBP-CI Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.6 30
-
 assay at Rattus norvegicus
1.5.1.6 30
-
 assay at Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Sus scrofa
1.5.1.6 NADP+
-
 Rattus norvegicus
1.5.1.6 NADP+
-
 Sus scrofa
1.5.1.6 tetrahydrofolate very tight binding Rattus norvegicus
1.5.1.6 tetrahydrofolate very tight binding Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.6 0.001
-
 tetrahydrofolate
-
 Sus scrofa
1.5.1.6 0.007
-
 tetrahydrofolate
-
 Rattus norvegicus