EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.6 | tetrahydrofolate | very strong product inhibition | Sus scrofa | |
1.5.1.6 | tetrahydropteroylpolyglutamate | inhibition increases with increasing length of polyglutamyl chain | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.6 | 0.0004 | - |
NADP+ | spectrophotometric assay | Sus scrofa | |
1.5.1.6 | 0.0006 | - |
NADP+ | fluorimetric assay | Sus scrofa | |
1.5.1.6 | 0.0044 | - |
10-formyltetrahydrofolate | racemic, fluorimetric assay | Sus scrofa | |
1.5.1.6 | 0.0075 | - |
10-formyltetrahydrofolate | racemic, spectrophotometric assay | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.1.6 | soluble | - |
Sus scrofa | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 92500 | - |
4 * 92500, SDS-PAGE | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | Sus scrofa | major disposal route for excess one-carbon units produced in folate-mediated metabolism | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.6 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.6 | - |
Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.6 | liver | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 0.64 | - |
- |
Sus scrofa |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.6 | -20°C, dialyzed against 0.01 M potassium phosphate buffer, pH 7.3, 40% glycerol, 0.1 M KCl, several weeks, stable | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | major disposal route for excess one-carbon units produced in folate-mediated metabolism | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
1.5.1.6 | 10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O | - |
Sus scrofa | tetrahydropteroylpolyglutamate + CO2 + NADPH + H+ | - |
? | |
1.5.1.6 | additional information | bifunctional enzyme: 10-formyltetrahydrofolate dehydrogenase separated from hydrolase activity, activities are functions of different active sites and take place simultaneously in presence of NADP+ | Sus scrofa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.6 | homotetramer | 4 * 92500, SDS-PAGE | Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 25 | - |
assay at | Sus scrofa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.6 | 1 | - |
10-formyltetrahydrofolate | - |
Sus scrofa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.6 | 10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Sus scrofa | |
1.5.1.6 | NADP+ | NADP+-dependent | Sus scrofa | |
1.5.1.6 | tetrahydrofolate | - |
Sus scrofa |