EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.6 | hydroxylamine | 0.1 M, strong inhibition | Sus scrofa | |
1.5.1.6 | iodoacetamide | inhibition reversed by 2-mercaptoethanol | Sus scrofa | |
1.5.1.6 | additional information | - |
Rattus norvegicus | |
1.5.1.6 | additional information | not inhibited by (+)-10-formyltetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, folate, aminopterin, tetrahydroaminopterin | Sus scrofa | |
1.5.1.6 | p-chloromercuribenzoate | inhibition reversed by 2-mercaptoethanol | Sus scrofa | |
1.5.1.6 | tetrahydrofolate | - |
Rattus norvegicus | |
1.5.1.6 | tetrahydrofolate | strong product inhibition by both isomers | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.6 | 0.0035 | - |
NADP+ | - |
Sus scrofa | |
1.5.1.6 | 0.0082 | - |
10-formyltetrahydrofolate | (-)-isomer | Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 320000 | - |
gel filtration | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.6 | Gallus gallus | - |
- |
- |
1.5.1.6 | Rattus norvegicus | - |
- |
- |
1.5.1.6 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.6 | - |
Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.6 | heart | less activity | Rattus norvegicus | - |
1.5.1.6 | kidney | less activity | Rattus norvegicus | - |
1.5.1.6 | liver | - |
Gallus gallus | - |
1.5.1.6 | liver | - |
Rattus norvegicus | - |
1.5.1.6 | liver | - |
Sus scrofa | - |
1.5.1.6 | spleen | less activity | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 0.98 | - |
- |
Sus scrofa |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.6 | -15°C, presence of 0.01 mM 2-mercaptoethanol, several weeks, stable | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
ir | |
1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | calculated equilibrium constant: 160000000, specific for (-)-10-formyltetrahydrofolate and NADP+ | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
ir | |
1.5.1.6 | additional information | - |
Rattus norvegicus | ? | - |
? | |
1.5.1.6 | additional information | not as substrate: (+)-10-formyltetrahydrofolate | Sus scrofa | ? | - |
? | |
1.5.1.6 | additional information | not as substrate: 5-formyltetrahydrofolate | Sus scrofa | ? | - |
? | |
1.5.1.6 | additional information | enzyme catalyzes hydrolytic cleavage of 10-formyltetrahydrofolate to formate and tetrahydrofolate in absence of NADP+ (15-30% of the rate of oxidative reaction) | Sus scrofa | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 30 | - |
assay at | Rattus norvegicus |
1.5.1.6 | 30 | - |
assay at | Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 7.8 | - |
- |
Rattus norvegicus |
1.5.1.6 | 7.8 | - |
broad pH-optimum centers at pH 7.8 | Sus scrofa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 6.5 | 8.5 | more than 60% of maximal activity at pH 6.5 and 8.5 | Sus scrofa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.6 | 10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
1.5.1.6 | 10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Sus scrofa | |
1.5.1.6 | NAD+ | less than 5% of the activity with NADP+ | Sus scrofa | |
1.5.1.6 | NADP+ | specific for | Rattus norvegicus | |
1.5.1.6 | NADP+ | specific for | Sus scrofa | |
1.5.1.6 | tetrahydrofolate | - |
Rattus norvegicus | |
1.5.1.6 | tetrahydrofolate | - |
Sus scrofa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.6 | 0.001 | - |
tetrahydrofolate | (-)-isomer | Sus scrofa | |
1.5.1.6 | 0.01 | - |
tetrahydrofolate | (+)-isomer | Sus scrofa |